Androgen down-regulated and region-specific expression of germ cell nuclear factor in mouse epididymis

	Androgen down-regulated and region-specific expression of germ cell nuclear factor in mouse epididymis
	Hu, YX; Zhou, ZY; Xu, C; Shang, Q; Zhang, YD; Zhang, YL
刊名	ENDOCRINOLOGY
	2003
卷号	144 期号:4 页码:1612-1619
通讯作者	Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,yonglz@sunm.shcnc.ac.cn
英文摘要	Germ cell nuclear factor (GCNF), a nuclear orphan receptor, involved in spermatogenesis, neurogenesis, differentiation, and embryo development, was highly expressed with two transcripts (7.4 and 2.3 kb) in mouse testis and with only one transcript (7.4 kb) slightly expressed in brain, liver, and kidney. The 2.3-kb transcript was restricted to round spermatids at stages VII and VIII of the spermatogenic cycle. The present report demonstrated its expression in epididymis as well, but at a very low level. Northern blot analysis showed two transcripts: a common 7.4-kb transcript and a unique 3.1-kb transcript. The expression levels of both GCNF transcripts in epididymis were down-regulated by androgen, as observed in castrated animals and aged mice. Polyclonal antisera against GCNF protein were raised. Western blot analysis showed the presence of only one band in total protein extracts from either mouse testis or epididymis. It indicated that the two mRNAs (7.4 and 3.1 kb) encode for the same protein as in testis. Fluorescent immunohistochemical staining and in situ hybridization showed that its expression was in the principal cell abundant in the corpus region. It implies that some androgen-regulated gene expressions located at the corpus principal cells might be controlled by GCNF.
学科主题	Endocrinology & Metabolism
类目[WOS]	Endocrinology & Metabolism
关键词[WOS]	ORPHAN RECEPTOR GCNF ; GENE-TRANSCRIPTION ; RAT EPIDIDYMIS ; CLONING ; TESTIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000182188900055
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2284]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, YX,Zhou, ZY,Xu, C,et al. Androgen down-regulated and region-specific expression of germ cell nuclear factor in mouse epididymis[J]. ENDOCRINOLOGY,2003,144(4):1612-1619.
APA	Hu, YX,Zhou, ZY,Xu, C,Shang, Q,Zhang, YD,&Zhang, YL.(2003).Androgen down-regulated and region-specific expression of germ cell nuclear factor in mouse epididymis.ENDOCRINOLOGY,144(4),1612-1619.
MLA	Hu, YX,et al."Androgen down-regulated and region-specific expression of germ cell nuclear factor in mouse epididymis".ENDOCRINOLOGY 144.4(2003):1612-1619.