The concentration of hydrogen peroxide generated during aggregation of alpha-synuclein in vitro is lower than 5 nmol/L

	The concentration of hydrogen peroxide generated during aggregation of alpha-synuclein in vitro is lower than 5 nmol/L
	Ji, LN; Li, HT; Luo, XY; Zhang, F; Hu, HY; Hu, J
刊名	CHINESE JOURNAL OF CHEMISTRY
	2004
卷号	22 期号:12 页码:1440-1443
关键词	alpha-synuclein hydrogen peroxide aggregation cytotoxicity
通讯作者	Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.,jhu@sjtu.edu.cn
英文摘要	Using a fluorometric method with a detection limit of 5 nmol/L, here it is reported that albeit positive results were got from bovine serum albumin (BSA) and chicken ovalbun-fin (OVA) as published in literature, no detectable amount of hydrogen peroxide (H2O2) was generated during alpha-synuclein (alpha-Syn) aggregation in vitro even in the presence of transition metal ions Cu(II) or Fe(III). The results suggest that the concentration of H2O2 generated during aggregation of alpha-Syn in vitro be lower than 5 nmol/L beyond the detection limit of the adopted method and it is far too poor to be responsible for the cytotoxicity of alpha-Syn aggregates, thus allowing people to extensively elucidate the mechanism underlying neurotoxicities of the aggregates formed by some amyloidogenic proteins.
学科主题	Chemistry
类目[WOS]	Chemistry, Multidisciplinary
关键词[WOS]	PARKINSONS-DISEASE ; BETA-SHEET ; CELL-DEATH ; PROTEINS ; SUPEROXIDE ; APOPTOSIS ; MUTANT ; BODY
收录类别	SCI
语种	英语
WOS记录号	WOS:000225724600012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2161]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ji, LN,Li, HT,Luo, XY,et al. The concentration of hydrogen peroxide generated during aggregation of alpha-synuclein in vitro is lower than 5 nmol/L[J]. CHINESE JOURNAL OF CHEMISTRY,2004,22(12):1440-1443.
APA	Ji, LN,Li, HT,Luo, XY,Zhang, F,Hu, HY,&Hu, J.(2004).The concentration of hydrogen peroxide generated during aggregation of alpha-synuclein in vitro is lower than 5 nmol/L.CHINESE JOURNAL OF CHEMISTRY,22(12),1440-1443.
MLA	Ji, LN,et al."The concentration of hydrogen peroxide generated during aggregation of alpha-synuclein in vitro is lower than 5 nmol/L".CHINESE JOURNAL OF CHEMISTRY 22.12(2004):1440-1443.