PEG10 Activation by Co-Stimulation of CXCR5 and CCR7 Essentially Contributes to Resistance to Apoptosis in CD19(+)CD34(+) B Cells from Patients with B Cell Lineage Acute and Chronic Lymphocytic Leukemia

	PEG10 Activation by Co-Stimulation of CXCR5 and CCR7 Essentially Contributes to Resistance to Apoptosis in CD19(+)CD34(+) B Cells from Patients with B Cell Lineage Acute and Chronic Lymphocytic Leukemia
	Hu, CS; Xiong, J; Zhang, LJ; Huang, BJ; Zhang, QP; Li, Q; Yang, MZ; Wu, Y; Wu, Q; Shen, Q
刊名	CELLULAR & MOLECULAR IMMUNOLOGY
	2004
卷号	1 期号:4 页码:280-294
关键词	leukemia B cells chemokine receptor apoptosis chemotaxis
通讯作者	Tan, JQ (reprint author), Wuhan Univ, Sch Med, Dept Immunol, Wuhan 430071, Peoples R China.,jinquan_tan@hotmail.com
英文摘要	We investigated CD19(+)CD34(+) and CD19(+)CD34(-) B cells from cord blood (CB) and typical patients with B cell lineage acute and chronic lymphocytic leukemia (B-ALL and B-CLL) in terms of expression and functions of CXCR5/CXCL13 and CCR7/CCL19. CXCR5 and CCR7 were selectively frequent expressed on B-ALL, B-CLL and CB CD19(+) CD34(+) B cells, but not on CD19(+)CD34(-) B cells. Instead of induction of impressive chemotactic responsiveness, CXCL13 and CCL19 together induced significant resistance to TNF-alpha-mediated apoptosis in B-ALL and B-CLL but not CB CD19(+) CD34(+) B cells. B-ALL and B-CLL CD19(+)CD34(+) B cells expressed elevated level of Paternally Expressed Gene 10 (PEG10), and CXCL13 and CCL19 together significantly up-regulated PEG10 expression in the cells. We found that CXCL13 and CCL19 together by means of activation of CXCR5 and CCR7 up-regulated PEG10 expression and function, subsequent stabilized caspase-3 and caspase-8 in B-ALL and B-CLL CD19(+)CD34(+) B cells, and rescued the cells from TNF-alpha-mediated apoptosis. We suggested that normal lymphocytes, especially naive B and T cells, utilized CXCR5/CXCL13 and CCR7/CCL19 for migration, homing, maturation, and cell homeostasis as well as secondary lymphoid tissues organogenesis. Meanwhile certain malignant cells took advantages of CXCR5/CXCL13 and CCR7/CCL19 for infiltration, resistance to apoptosis, and inappropriate proliferation.
学科主题	Immunology
类目[WOS]	Immunology
收录类别	SCI
语种	英语
WOS记录号	WOS:000208923500006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2069]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, CS,Xiong, J,Zhang, LJ,et al. PEG10 Activation by Co-Stimulation of CXCR5 and CCR7 Essentially Contributes to Resistance to Apoptosis in CD19(+)CD34(+) B Cells from Patients with B Cell Lineage Acute and Chronic Lymphocytic Leukemia[J]. CELLULAR & MOLECULAR IMMUNOLOGY,2004,1(4):280-294.
APA	Hu, CS.,Xiong, J.,Zhang, LJ.,Huang, BJ.,Zhang, QP.,...&Tan, JQ.(2004).PEG10 Activation by Co-Stimulation of CXCR5 and CCR7 Essentially Contributes to Resistance to Apoptosis in CD19(+)CD34(+) B Cells from Patients with B Cell Lineage Acute and Chronic Lymphocytic Leukemia.CELLULAR & MOLECULAR IMMUNOLOGY,1(4),280-294.
MLA	Hu, CS,et al."PEG10 Activation by Co-Stimulation of CXCR5 and CCR7 Essentially Contributes to Resistance to Apoptosis in CD19(+)CD34(+) B Cells from Patients with B Cell Lineage Acute and Chronic Lymphocytic Leukemia".CELLULAR & MOLECULAR IMMUNOLOGY 1.4(2004):280-294.