Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells

	Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells
	Gong, YQ; Fan, Y; Liu, L; Wu, DZ; Chang, ZL; Wang, ZT
刊名	IN VIVO
	2004
卷号	18 期号:2 页码:223-228
关键词	erianin endothelial cells acidification rate metabolism JNK/SAPK
通讯作者	Wang, ZT (reprint author), Shanghai Univ Trad Chinese Med, Inst Chinese Mat Med, 1200 Cailun Rd,Zhangijang Hi Tech Pk, Shanghai 201203, Peoples R China.,wangzht@shutcm.edu.cn
英文摘要	Background Erianin is a natural product derived from Dendrobium chrysotoxum, with promising antitumor activity. Materials and Methods: To evaluate the metabolic effect of erianin, a cytosensor assay for acidification rate, MTT assay, measurement of lactate, glucose and ATP were performed in human umbilical vein endothelial cells (HUVECs) exposed to 1100 nM erianin. JNK/SAPK activity was detected by Western blot. Results: Twelve- or 24- hour incubation with erianin induced a dose-dependent metabolic inhibition, as indicated by reduced acidification rate and cell viability, with an endothelium-selectivity. Erianin caused decreases in lactate production, glucose consumption and intracellular ATP level. Pretreatment with the JNK/SAPK inhibitor SP600125 significantly abolished these inhibitory responses, and especially restored the erianin-induced decreases in ATP and the erianin-induced phosphorylation of JNK/SAPK with dose- and time- dependence. Conclusion: Erianin inhibited endothelial metabolism in a JNK/SAPK-dependent manner. This mechanism may be involved in the potential antitumor and antiangiogenic actions of erianin.
学科主题	Research & Experimental Medicine
类目[WOS]	Medicine, Research & Experimental
关键词[WOS]	HUMAN TUMOR-CELLS ; IN-VITRO ; APOPTOSIS ; ACTIVATION ; HYPOXIA ; PATHWAY ; JNK ; TECHNOLOGY ; BIBENZYL ; STRESS
收录类别	SCI
语种	英语
WOS记录号	WOS:000220784000016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2038]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gong, YQ,Fan, Y,Liu, L,et al. Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells[J]. IN VIVO,2004,18(2):223-228.
APA	Gong, YQ,Fan, Y,Liu, L,Wu, DZ,Chang, ZL,&Wang, ZT.(2004).Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells.IN VIVO,18(2),223-228.
MLA	Gong, YQ,et al."Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells".IN VIVO 18.2(2004):223-228.