Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1)

	Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1)
	Hu, JH; Zhang, JF; Ma, YH; Jiang, J; Yang, N; Li, XB; Chi, ZGY; Fei, J; Guo, LH
刊名	CELL RESEARCH
	2004
卷号	14 期号:1 页码:54-59
关键词	GABA GAT1 testes sperm reproduction transgenic
通讯作者	Fei, J (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,mhzhang@sunm.shcne.ac.cn
英文摘要	It is well documented that gamma-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABA(A) and GABA(B) receptors were present in testis and sperm, and might mediate the acrosome reaction induced by GABA and progesterone. GABA transporter I (GAT1) also existed in testis and sperm, but its physiological function was unknown. In the present study, we used GAT1 overexpressing mice to explore GAT1 function in male reproductive system. We found that the expression level of GAT1 continuously increased in wild-type mouse testis from 1 month to 2 months after birth. GAT1 overexpression in mouse affected testis development, which embodied reduced testis mass and slowed spermatogenesis in transgenic mice. Moreover, transgenic mice showed increase of the percentage of broken sperm. The further study revealed that the reproductive capacity was impaired in GAT1 overexpressing mice. In addition, testosterone level was significantly low in transgenic mice compared with that in wild-type mice. Our findings provided the first evidence that abnormal expression of GAT1 could result in dysgenesis, and indicated that GAT1 might be therapeutically targeted for contraception or dysgenesis treatment.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	SPERM ACROSOME REACTION ; HUMAN SPERMATOZOA ; GABA TRANSPORTER ; RAT TESTIS ; RECEPTOR ; BRAIN ; IDENTIFICATION ; PROGESTERONE
收录类别	SCI
语种	英语
WOS记录号	WOS:000220195100007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2016]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, JH,Zhang, JF,Ma, YH,et al. Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1)[J]. CELL RESEARCH,2004,14(1):54-59.
APA	Hu, JH.,Zhang, JF.,Ma, YH.,Jiang, J.,Yang, N.,...&Guo, LH.(2004).Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1).CELL RESEARCH,14(1),54-59.
MLA	Hu, JH,et al."Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1)".CELL RESEARCH 14.1(2004):54-59.