Solution structure of the ubiquitin-like domain of human DC-UbP from dendritic cells

	Solution structure of the ubiquitin-like domain of human DC-UbP from dendritic cells
	Gao, YG; Song, AX; Shi, YH; Chang, YG; Liu, SX; Yu, YZ; Cao, XT; Lin, DH; Hu, HY
刊名	PROTEIN SCIENCE
	2005
卷号	14 期号:8 页码:2044-2050
关键词	solution structure dynamics ubiquitin-like domain DC-UbP NMR
通讯作者	Hu, HY (reprint author), Shanghai Inst Mat Med, Shanghai Inst Biol Sci, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China.,dhlin@mail.shcnc.ac.cn
英文摘要	The previously identified dendritic cell-derived ubiquitin-like protein (DC-UbP) was implicated in cellular differentiation and apoptosis. Sequence alignment suggested that it contains a ubiquitin-like (UbL) domain in the C terminus. Here, we present the solution NMR structure and backbone dynamics of the UbL domain of DC-UbP. The overall structure of the domain is very similar to that of Ub despite low similarity (< 30%) in amino-acid sequence. One distinct feature of the domain structure is its highly positively charged surface that is different from the corresponding surfaces of the well-known UbL modifiers, Ub, NEDD8, and SUMO-1. The key amino-acid residues responsible for guiding polyubiquitinated proteins to proteasome degradation in Ub are not conserved in the UbL domain. This implies that the UbL domain of DC-UbP may have its own specific interaction partners with other yet unknown cellular functions related to the Ub pathway.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	N-15 NMR RELAXATION ; CONJUGATION SYSTEM ; CHEMICAL-SHIFT ; PROTEIN ; FAMILY ; SUMO ; DESTRUCTION ; PROTEASOME ; RESTRAINTS ; AUTOPHAGY
收录类别	SCI
语种	英语
WOS记录号	WOS:000230872900011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1946]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gao, YG,Song, AX,Shi, YH,et al. Solution structure of the ubiquitin-like domain of human DC-UbP from dendritic cells[J]. PROTEIN SCIENCE,2005,14(8):2044-2050.
APA	Gao, YG.,Song, AX.,Shi, YH.,Chang, YG.,Liu, SX.,...&Hu, HY.(2005).Solution structure of the ubiquitin-like domain of human DC-UbP from dendritic cells.PROTEIN SCIENCE,14(8),2044-2050.
MLA	Gao, YG,et al."Solution structure of the ubiquitin-like domain of human DC-UbP from dendritic cells".PROTEIN SCIENCE 14.8(2005):2044-2050.