Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis

	Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis
	Cai, XH; Zhang, Q; Shi, SY; Ding, DF
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2005
卷号	37 期号:5 页码:293-302
关键词	two-component system phosphorelay histidine kinase response regulator three-dimensional cluster analysis
通讯作者	Ding, DF (reprint author), Chinese Acad Sci, Key Lab Proteom, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,Grad Sch, Shanghai 200031, Peoples R China.,dingdafu@server.shcnc.ac.cn
英文摘要	Two-component and phosphorelay signal transduction systems are central components in the virulence and antimicrobial resistance responses of a number of bacterial and fungal pathogens; in some cases, these systems are essential for bacterial growth and viability. Herein, we analyze in detail the conserved surface residue clusters in the phosphotransferase domain of histidine kinases and the regulatory domain of response regulators by using complex structure-based three-dimensional cluster analysis. We also investigate the protein-protein interactions that these residue clusters participate in. The Spo0B-Spo0F complex structure was used as the reference structure, and the multiple aligned sequences of phosphotransferases and response regulators were paired correspondingly. The results show that a contiguous conserved residue cluster is formed around the active site, which crosses the interface of histidine kinases and response regulators. The conserved residue clusters of phosphotransferase and the regulatory domains are directly involved in the functional implementation of two-component signal transduction systems and are good targets for the development of novel antimicrobial agents.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	RESPONSE REGULATOR PHOSPHOTRANSFERASE ; MOLECULAR RECOGNITION ; ESCHERICHIA-COLI ; GLYCOPEPTIDE RESISTANCE ; BACTERIAL CHEMOTAXIS ; PROTEIN CHEY ; ACTIVE-SITE ; PHOSPHORYLATION ; PHOSPHATASE ; MECHANISM
收录类别	SCI
语种	英语
WOS记录号	WOS:000229304400002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1914]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cai, XH,Zhang, Q,Shi, SY,et al. Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(5):293-302.
APA	Cai, XH,Zhang, Q,Shi, SY,&Ding, DF.(2005).Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(5),293-302.
MLA	Cai, XH,et al."Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.5(2005):293-302.