Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers

	Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers
	Li, C; Tan, YX; Zhou, H; Ding, SJ; Li, SJ; Ma, DJ; Man, XB; Hong, Y; Zhang, L; Li, L
刊名	PROTEOMICS
	2005
卷号	5 期号:4 页码:1125-1139
关键词	bioinformatics analysis hepatocellular carcinoma associated hepatitis B virus mass spectrometry proteome tumor markers two-dimensional gel electrophoresis
通讯作者	Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Res Ctr Proteome Anal, Inst Biochem & Cell Biol,Key Lab Proteom, 320 YueYang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn
英文摘要	Hepatocellular carcinoma (HCC) is a malignancy of both underdeveloped and developing countries. Proteomes of ten pairs of clinical hepatitis B virus associated HCC tissue samples were obtained by high resolution two-dimensional gel electrophoresis. Comprehensive analyses of proteins associated with B-type HCC were focused on total differentially expressed proteins (>= two-fold increase or decrease, Student's t-test, p < 0.05) from one pair of samples. Protein identification was done by peptide mass fingerprinting with matrix assisted laser desorption/ ionization-time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry Comparative analyses of proteins associated with B-type HCC included repeat statistics in ten cases. A total of 100 protein spots, corresponding to 80 different gene products, were identified. Proteins whose expression levels were different by more than 2-fold in at least 50% of the cases (five of ten cases) were further analyzed and 45 proteins were selected out as candidates for HCC-associated proteins. Western blotting further validated up-regulated expressions of two candidate proteins in tumor tissues: proliferating cell antigen and stathmin 1. This comprehensive and comparative analyses of proteins associated with B-type HCC could provide useful molecular markers for diagnostics and prognostics and for therapeutic targets. The physiological significance of the differential expressions for several candidate proteins are discussed.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
关键词[WOS]	DIFFERENCE GEL-ELECTROPHORESIS ; PRIMARY LIVER-CANCER ; MEMBRANE-PROTEINS ; MASS-SPECTROMETRY ; 2-DIMENSIONAL ELECTROPHORESIS ; GENE-EXPRESSION ; ALDEHYDE DEHYDROGENASE ; NUCLEAR-MEMBRANE ; MITOTIC SPINDLE ; HUMAN HEPATOMA
收录类别	SCI
语种	英语
WOS记录号	WOS:000227909800030
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1905]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, C,Tan, YX,Zhou, H,et al. Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers[J]. PROTEOMICS,2005,5(4):1125-1139.
APA	Li, C.,Tan, YX.,Zhou, H.,Ding, SJ.,Li, SJ.,...&Zeng, R.(2005).Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers.PROTEOMICS,5(4),1125-1139.
MLA	Li, C,et al."Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers".PROTEOMICS 5.4(2005):1125-1139.