Effective gene-virotherapy tumor mediated by the for complete eradication of combination of hTRAIL (TNFSF10) and plasminogen k5

	Effective gene-virotherapy tumor mediated by the for complete eradication of combination of hTRAIL (TNFSF10) and plasminogen k5
	Liu, XY; Qiu, SB; Zou, WG; Pei, ZF; Gu, JF; Luo, CX; Ruan, HM; Chen, Y; Qi, YP; Qian, C
刊名	MOLECULAR THERAPY
	2005
卷号	11 期号:4 页码:531-541
关键词	cancer gene therapy oncolytic adenovirus gene-virotherapy ZD55 hTRAIL k5 apoptosis angiogenesis
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn ; qiyipeng@whu.edu.cn ; cqian@unav.es
英文摘要	Virotherapy with oncolytic viruses is a highly promising approach for cancer therapy. To improve further the therapeutic effect of oncolytic viruses, therapeutic genes have been incorporated into these types of vectors. In this study, we have inserted hTRAIL (approved gene symbol TNFSF10) into the ZD55 vector, which was based on deletion of the adenoviral E1B 55-kDa gene and could replicate in and lyse p53-deficient tumors. Our data shows that infection of colorectal carcinoma cells with ZD55-hTRAIL resulted in tumor cell death that was much greater than that induced by ZD55 vector or replication-defective adenovirus expressing hTRAIL. In contrast to these, ZD55-hTRAIL did not induce any cytopathic effect in normal cells. Treatment of established tumor with ZD55-hTRAIL resulted in dramatic inhibition of tumor growth in an animal model of colorectal carcinoma. However, when the established tumors were treated by coadministration of ZD55h-TRAIL and Ad-k5, we observed complete eradication of the established tumors in all animals treated with the combined therapy. This strong anti-tumor activity was due to the fact that two genes may act with compensative (or synergic) effect through different mechanisms to kill tumors. Therefore, targeting dual gene-virotherapy may be one of the best strategies for cancer therapy if two suitable genes are chosen.
学科主题	Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine
类目[WOS]	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
关键词[WOS]	SELECTIVELY-REPLICATING ADENOVIRUS ; TRAIL-INDUCED APOPTOSIS ; HERPES-SIMPLEX-VIRUS ; ANTITUMOR-ACTIVITY ; IN-VIVO ; HEPATOCELLULAR-CARCINOMA ; DELETED ADENOVIRUS ; INTRATUMORAL COINJECTION ; CHEMOTHERAPEUTIC-AGENTS ; COLORECTAL-CARCINOMA
收录类别	SCI
语种	英语
WOS记录号	WOS:000227991700008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1899]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, XY,Qiu, SB,Zou, WG,et al. Effective gene-virotherapy tumor mediated by the for complete eradication of combination of hTRAIL (TNFSF10) and plasminogen k5[J]. MOLECULAR THERAPY,2005,11(4):531-541.
APA	Liu, XY.,Qiu, SB.,Zou, WG.,Pei, ZF.,Gu, JF.,...&Qian, C.(2005).Effective gene-virotherapy tumor mediated by the for complete eradication of combination of hTRAIL (TNFSF10) and plasminogen k5.MOLECULAR THERAPY,11(4),531-541.
MLA	Liu, XY,et al."Effective gene-virotherapy tumor mediated by the for complete eradication of combination of hTRAIL (TNFSF10) and plasminogen k5".MOLECULAR THERAPY 11.4(2005):531-541.