A novel short-chain peptide BmKX from the chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination

	A novel short-chain peptide BmKX from the chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination
	Wang, CG; Cai, Z; Lu, WY; Wu, JH; Xu, YQ; Shi, YY; Chi, CW
刊名	TOXICON
	2005
卷号	45 期号:3 页码:309-319
关键词	scorpion toxin gene structure cysteine framework NMR solution structure
通讯作者	Shi, YY (reprint author), Univ Sci & Technol China, Sch Life Sci, Struct Biol Lab, Hefei 230026, Anhui, Peoples R China.,yyshi@ustc.edu.cn ; chi@sunm.shen-c.ac.cn
英文摘要	Scorpion venom is a rich source of bioactive peptides. From the venom of Chinese scorpion Buthus martensi Karsch (BmK), a novel short chain peptide BmKX of 31-amino acid residues was purified, and its amino acid sequence and gene structure were determined. The gene of BmKX was composed of two exons interrupted by an 86-bp intron at the codon-7 upstream of the mature peptide. Although its gene structure is similar to those of other known scorpion toxins, its amino acid sequence, especially the cysteine framework, is different from those of all other known subfamilies of short-chain scorpion toxins. The solution structure of BmKX, determined with two-dimensional NMR spectroscopy, shows that BmKX also forms a typical cysteine-stabilized alpha/beta scaffold adopted by most short-chain scorpion toxins, consisting of a short 3(10)-hefix and a two-stranded antiparallel beta-sheet, and the short N-terminal segment forms a pseudo-strand of the beta-sheet. However, the orientation between the helix and the beta-sheet is significantly different from the others, which might be the reason for its unique but still unclear physiological function. (C) 2004 Elsevier Ltd. All rights reserved.
学科主题	Pharmacology & Pharmacy; Toxicology
类目[WOS]	Pharmacology & Pharmacy ; Toxicology
关键词[WOS]	3-DIMENSIONAL STRUCTURE ; POTASSIUM CHANNELS ; MOLECULAR CHARACTERIZATION ; GENOMIC ORGANIZATION ; TITYUS-SERRULATUS ; K+ CHANNEL ; TOXINS ; NEUROTOXINS ; PROTEIN ; VENOM
收录类别	SCI
语种	英语
WOS记录号	WOS:000227029800008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1885]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, CG,Cai, Z,Lu, WY,et al. A novel short-chain peptide BmKX from the chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination[J]. TOXICON,2005,45(3):309-319.
APA	Wang, CG.,Cai, Z.,Lu, WY.,Wu, JH.,Xu, YQ.,...&Chi, CW.(2005).A novel short-chain peptide BmKX from the chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination.TOXICON,45(3),309-319.
MLA	Wang, CG,et al."A novel short-chain peptide BmKX from the chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination".TOXICON 45.3(2005):309-319.