Expression of trophinin and bystin identifies distinct cell types in the germinal zones of adult rat brain

	Expression of trophinin and bystin identifies distinct cell types in the germinal zones of adult rat brain
	Ma, LP; Yin, M; Wu, XF; Wu, CH; Yang, S; Sheng, JS; Ni, HJ; Fukuda, MN; Zhou, JW
刊名	EUROPEAN JOURNAL OF NEUROSCIENCE
	2006
卷号	23 期号:9 页码:2265-2276
关键词	astrocytes neuroblasts neurogenesis rostral migratory stream subventricular zone
通讯作者	Zhou, JW (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,jwzhou@sibs.ac.cn
英文摘要	In the adult brain, the subventricular zone (SVZ) is one of the regions where active neurogenesis occurs. Relatively few specific markers are available to distinguish different types of cells in the SVZ and rostral migratory stream (RMS) of adult brain. Here, we showed that trophinin and bystin, both of which are required for early embryo implantation during development, were expressed in the SVZ and RMS of the adult rat brain, but not in the brain of embryos and early postnatal animals. Trophinin-expressing cells were immunopositive for both Ki-67 and nestin in the SVZ. Some of the trophinin-positive cells did not express either the type A cell marker polysialylated weakly adhesive form of the neural cell adhesion molecule (PSA-NCAM) or the type B cell marker glial fibrillary acidic protein (GFAP). Double-label immunohistochemistry revealed that bystin-positive cells co-expressed GFAP, Ki-67 and nestin, but not PSA-NCAM, suggesting that they are likely type B cells. Intracerebroventricular infusion of cytosine-beta-D-arabiofuranoside (Ara-C) eliminated trophinin-positive cells in the SVZ. Following its depletion, however, the remaining bystin-positive cells continued to divide and generate actively dividing trophinin-positive cells that were negative for PSA-NCAM, leading to reconstruction of SVZ network. These characteristics indicate that this subset of trophinin-positive cells in the SVZ is type C cells. Conversely in the RMS, trophinin co-localized with nestin and PSA-NCAM, suggesting that it is expressed in neuroblasts. Cultured neural precursor cells derived from the adult SVZ also expressed both trophinin and bystin. These findings provide insight into the molecular basis of adult neurogenesis in the SVZ and RMS.
学科主题	Neurosciences & Neurology
类目[WOS]	Neurosciences
关键词[WOS]	NEURAL STEM-CELLS ; ENDOMETRIAL EPITHELIAL-CELLS ; SUBVENTRICULAR ZONE ; MAMMALIAN BRAIN ; POSTNATAL NEUROGENESIS ; OLFACTORY-BULB ; CYTOPLASMIC PROTEIN ; MOUSE BRAIN ; SPINAL-CORD ; MIGRATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000237603500003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1763]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ma, LP,Yin, M,Wu, XF,et al. Expression of trophinin and bystin identifies distinct cell types in the germinal zones of adult rat brain[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2006,23(9):2265-2276.
APA	Ma, LP.,Yin, M.,Wu, XF.,Wu, CH.,Yang, S.,...&Zhou, JW.(2006).Expression of trophinin and bystin identifies distinct cell types in the germinal zones of adult rat brain.EUROPEAN JOURNAL OF NEUROSCIENCE,23(9),2265-2276.
MLA	Ma, LP,et al."Expression of trophinin and bystin identifies distinct cell types in the germinal zones of adult rat brain".EUROPEAN JOURNAL OF NEUROSCIENCE 23.9(2006):2265-2276.