Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp) | |
Jin, XL; Tao, ZJ; Jia, J; He, XX; Jin, YX | |
刊名 | CHINESE SCIENCE BULLETIN |
2006 | |
卷号 | 51期号:7页码:824-829 |
关键词 | species-specific aminoacylation tRNA(Trp) tryptophanyl-tRNA synthetase (TrpRS) O. sativa mitochondrial |
通讯作者 | He, XX (reprint author), Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China.,jhhxx@zjnu.cn ; yxjin@sibs.ac.cn |
英文摘要 | The details of species-specific aminoacylation in Oryza sativa mitochondrial tRNA(TrP) by bacterial and eukaryotic (cytoplasm) tryptophanyl-tRNA synthetases (TrpRS) were investigated. Seven single or multiple mutations of three bases (G73, U72, A 68) were made in O.sativa mitochondrial tRNA(Trp) to the corresponding nucleotides present in human tRNA(Trp). In vitro transcripts of these mutant genes were tryptophanylated by Bacillus subtilis and human tryptophanyl-tRNA synthetases (TrpRS), and the kinetic parameters were determined. The results showed that the aminoacylation of seven mutant transcripts by B. subtilis TrpRS was 53.33%-99.79% less efficient than that by wild-type O.sativa mitochondrial tRNA(Trp), but was 4-330 times more efficient than that by human TrpRS. The mutant MPH7 (G73, U72 and C68 in O.sativa mitochondrial tRNA were all replaced by the counterpart residues from human tRNA(Trp) and showed a great change in aminoacylation efficiency. Our results inclicate that the species-specific identity elements of O.sativa mitochondrial tRNA(Trp) are similar to bacterial and eukaryotic (cytoplasm). They are mainly located at the discriminator base, the first and the fifth pairs of bases, the discriminator base G73, two bases in the acceptor stem G1/U72 and U5/A68. Our results also provide new data in support of the hypothesis that mitochondrial tRNA Trp is of eubacterial origin. |
学科主题 | Science & Technology - Other Topics |
类目[WOS] | Multidisciplinary Sciences |
关键词[WOS] | TRANSFER-RNA-SYNTHETASE ; BACILLUS-SUBTILIS ; IDENTITY ; RECOGNITION ; RULES ; CODE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000237493500010 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/1743] |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Jin, XL,Tao, ZJ,Jia, J,et al. Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp)[J]. CHINESE SCIENCE BULLETIN,2006,51(7):824-829. |
APA | Jin, XL,Tao, ZJ,Jia, J,He, XX,&Jin, YX.(2006).Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp).CHINESE SCIENCE BULLETIN,51(7),824-829. |
MLA | Jin, XL,et al."Species-specific aminoacylation of Oryza sativa mitochondrial tRNA(Trp)".CHINESE SCIENCE BULLETIN 51.7(2006):824-829. |
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