Regulated expression of TAF1 in 1-cell mouse embryos

	Regulated expression of TAF1 in 1-cell mouse embryos
	Wang, K; Sun, F; Sheng, HZ
刊名	ZYGOTE
	2006
卷号	14 期号:3 页码:209-215
关键词	TBP TAF1 ZGA
通讯作者	Sheng, HZ (reprint author), Shanghai Jiao Tong Univ, Sch Med, Ctr Dev Biol, Xinhua Hosp, 1665 Kong Jiang Rd, Shanghai 200092, Peoples R China.,hzsheng2003@yahoo.com
英文摘要	TATA binding protein (TBP) associated factor 1 (TAF1) is a member of the general transcription machinery. Interference in the function of TAF1 causes a broad transcriptional defect in early development. To explore possible roles of TAF1 in embryonic transcriptional silence and zygotic genome activation, we examined the expression of TAF1 in 1-cell mouse embryos. Using an immunofluorescence assay, TAF1 was not detected in embryos in the first few hours after fertilization. TAF1 appeared in pronuclei 6 h post-fertilization and reached a relatively high level before zygotic genome activation. These data show that besides TBP, another critical member of the general transcription machinery such as TAF1 is also absent or at an extremely low level at the outset of development. Combined deficiency in critical members of the general transcription machinery may account for embryonic transcriptional silence.
学科主题	Cell Biology; Developmental Biology; Reproductive Biology
类目[WOS]	Cell Biology ; Developmental Biology ; Reproductive Biology
关键词[WOS]	BOX-BINDING PROTEIN ; MAJOR DEVELOPMENTAL TRANSITION ; EARLY XENOPUS-EMBRYOS ; GENE-EXPRESSION ; TRANSCRIPTIONAL ACTIVITY ; PREIMPLANTATION EMBRYO ; STAGE
收录类别	SCI
语种	英语
WOS记录号	WOS:000239106300004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1681]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, K,Sun, F,Sheng, HZ. Regulated expression of TAF1 in 1-cell mouse embryos[J]. ZYGOTE,2006,14(3):209-215.
APA	Wang, K,Sun, F,&Sheng, HZ.(2006).Regulated expression of TAF1 in 1-cell mouse embryos.ZYGOTE,14(3),209-215.
MLA	Wang, K,et al."Regulated expression of TAF1 in 1-cell mouse embryos".ZYGOTE 14.3(2006):209-215.