Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dyenin-dependent and -independent centrosomal protein assembly

	Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dyenin-dependent and -independent centrosomal protein assembly
	Guo, J; Yang, ZY; Song, W; Chen, Q; Wang, FB; Zhang, QG; Zhu, XL
刊名	MOLECULAR BIOLOGY OF THE CELL
	2006
卷号	17 期号:2 页码:680-689
通讯作者	Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn
英文摘要	The centrosome is the major microtubule-organizing center in animal cells. Although the cytoplasmic dynein regulator Nudel interacts with centrosomes, its role herein remains unclear. Here, we show that in Cos7 cells Nudel is a mother centriole protein with rapid turnover independent of dynein activity. During centriole duplication, Nudel targets to the new mother centriole later than ninein but earlier than dynactin. Its centrosome localization requires a C-terminal region that is essential for associations with dynein, dynactin, pericentriolar material (PCM)-1, pericentrin, and gamma-tubulin. Overexpression of a mutant Nudel lacking this region, a treatment previously shown to inactivate dynein, dislocates centrosomal Lis1, dynactin, and PCM-1, with little influence on pericentrin and gamma-tubulin in Cos7 and HeLa cells. Silencing Nudel in HeLa cells markedly decreases centrosomal targeting of all the aforementioned proteins. Silencing Nudel also represses centrosomal NIT nucleation and anchoring. Furthermore, Nudel can interact with pericentrin independently of dynein. Our current results suggest that Nudel plays a role in both dynein-mediated centripetal transport of dynactin, Lis1, and PCM-1 as well as in dynein-independent centrosomal targeting of pericentrin and gamma-tubulin. Moreover, Nudel seems to tether dynactin and dynein to the mother centriole for MT anchoring.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	CYTOPLASMIC DYNEIN ; CELL-CYCLE ; GAMMA-TUBULIN ; SPINDLE ORGANIZATION ; MITOTIC SPINDLE ; MOLECULAR CHARACTERIZATION ; NEURONAL MIGRATION ; DYNACTIN ; LIS1 ; PERICENTRIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000235117300012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1673]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Guo, J,Yang, ZY,Song, W,et al. Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dyenin-dependent and -independent centrosomal protein assembly[J]. MOLECULAR BIOLOGY OF THE CELL,2006,17(2):680-689.
APA	Guo, J.,Yang, ZY.,Song, W.,Chen, Q.,Wang, FB.,...&Zhu, XL.(2006).Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dyenin-dependent and -independent centrosomal protein assembly.MOLECULAR BIOLOGY OF THE CELL,17(2),680-689.
MLA	Guo, J,et al."Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dyenin-dependent and -independent centrosomal protein assembly".MOLECULAR BIOLOGY OF THE CELL 17.2(2006):680-689.