Proteomic profiling of regionalized proteins in rat epididymis indicates consistency between specialized distribution and protein functions

	Proteomic profiling of regionalized proteins in rat epididymis indicates consistency between specialized distribution and protein functions
	Yuan, HX; Zhang, L; Liu, AH; Zhou, H; Wang, YG; Zhang, H; Wang, GQ; Zeng, R; Zhang, YL; Chen, ZJ
刊名	JOURNAL OF PROTEOME RESEARCH
	2006
卷号	5 期号:2 页码:299-307
关键词	rat epididymis two-dimensional gel electrophoresis regional distribution inducible carbonyl reductase androgen
通讯作者	Zhang, YL (reprint author), Shanghai Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn ; zjchen@sibs.ac.cn
英文摘要	The epididymis is a key structure of the male reproductive system; its function is to mature, transport, and store sperm. Most of the research examining the epididymis to date has been limited to the study of the secreted proteins involved in the maturation of spermatozoa. However, it is also very important to understand the protein components, regulation and function of the tissue itself since these are the basis for all of its physiological processes. We investigated the differential expression of proteins among the caput, corpus, and cauda regions of rat epididymis and considered the possible links between the localization of these proteins and the different functions of these epididymal regions. High-resolution 2-D gel electrophoresis followed by mass spectrometry (MS) revealed 28 distinct proteins whose expression levels varied from the Caput to the cauda epididymis. Sixteen of them were reported for first time to be expressed in the epididymis. Expression patterns of some proteins were validated by Northern blot or Western blot. Immunohistochemistry revealed that inducible carbonyl reductase (iCR), an important enzyme in the anti-oxidative system, exhibits primary and cell-type specific distribution in the distal cauda region. Moreover, analysis of iCR transcription in castrated animals showed that its expression is androgen-dependent. Together with its known functions, iCR may also be involved in androgen metabolism and maintaining a steady microenvironment in the duct of epididymis.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	VAS-DEFERENS ; NUCLEAR-LOCALIZATION ; CARBONYL REDUCTASE ; HEPATOCELLULAR-CARCINOMA ; GENE-EXPRESSION ; MESSENGER-RNA ; GENITAL-TRACT ; LUMINAL FLUID ; HUMAN-TISSUES ; IDENTIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000235317600010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1672]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yuan, HX,Zhang, L,Liu, AH,et al. Proteomic profiling of regionalized proteins in rat epididymis indicates consistency between specialized distribution and protein functions[J]. JOURNAL OF PROTEOME RESEARCH,2006,5(2):299-307.
APA	Yuan, HX.,Zhang, L.,Liu, AH.,Zhou, H.,Wang, YG.,...&Chen, ZJ.(2006).Proteomic profiling of regionalized proteins in rat epididymis indicates consistency between specialized distribution and protein functions.JOURNAL OF PROTEOME RESEARCH,5(2),299-307.
MLA	Yuan, HX,et al."Proteomic profiling of regionalized proteins in rat epididymis indicates consistency between specialized distribution and protein functions".JOURNAL OF PROTEOME RESEARCH 5.2(2006):299-307.