Enhanced sensitivity of hepatocellular carcinoma cells to chemotherapy with a Smac-armed oncolytic adenovirus

	Enhanced sensitivity of hepatocellular carcinoma cells to chemotherapy with a Smac-armed oncolytic adenovirus
	Pan, QW; Zhong, SY; Liu, BS; Liu, J; Cai, R; Wang, YG; Liu, XY; Qian, C
刊名	ACTA PHARMACOLOGICA SINICA
	2007
卷号	28 期号:12 页码:1996-2004
关键词	oncolytic adenovirus inhibitor of apoptosis proteins second mitochondria-derived activator of caspases chemotherapy hepato-cellular carcinoma
通讯作者	Liu, XY (reprint author), Zhejiang Sci Tech Univ, Life Sci Coll, Xin Yuan Inst Med & Biotechnol, Hangzhou 310018, Peoples R China.,xyliu@sibs.ac.cn ; cqian3182@sina.com.cn
英文摘要	Aim: The aim of the present study was to further improve the therapeutic effects for human hepatocellular carcinoma (HCC) and reduce the damage in normal cells using a novel chemo-gene-virotherapeutic strategy. Methods: An oncolytic adenoviral vector (ZD55) similar to the typical oncolytic adenovirus ONYX-015, with a deletion of E1B-55K gene, was employed to express the second mitochondria-derived activator of caspases (Smac) protein by constructing a recombinant virus ZD55-Smac. The enhanced cytotoxicity of the combined treatment of ZD55-Smac with cisplatin or 5-fluorouracil (5-FU) was evaluated in several HCC cell lines. Moreover, the negative effects on normal cells have been tested in human normal liver cell lines L-02 and QSG-7701 cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and apoptotic cell staining. Results: According to our observation, ZD55-Smac is superior to ONYX-015 in sensitizing chemotherapy, ZD55-Smac used in conjunction with chemotherapy was found to exhibit obviously enhanced cytotoxicity in HCC cells, yet significantly abolished the negative toxicity in normal cells by utilizing the tumor selective replication vector and reducing the dosage. Conclusion: This chemo-gene-virotherapeutic (cisplatin or 5-FU+ZD55-Smac) strategy is superior to the conventional chemo-gene or chemo-viro approach.
学科主题	Chemistry; Pharmacology & Pharmacy
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
关键词[WOS]	LOW-DOSE CISPLATIN ; CANCER-CELLS ; ANTITUMOR-ACTIVITY ; STRUCTURAL BASIS ; HUMAN HEPATOMA ; IN-VIVO ; APOPTOSIS ; XIAP ; EXPRESSION ; ONYX-015
收录类别	SCI
语种	英语
WOS记录号	WOS:000251327800018
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1618]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Pan, QW,Zhong, SY,Liu, BS,et al. Enhanced sensitivity of hepatocellular carcinoma cells to chemotherapy with a Smac-armed oncolytic adenovirus[J]. ACTA PHARMACOLOGICA SINICA,2007,28(12):1996-2004.
APA	Pan, QW.,Zhong, SY.,Liu, BS.,Liu, J.,Cai, R.,...&Qian, C.(2007).Enhanced sensitivity of hepatocellular carcinoma cells to chemotherapy with a Smac-armed oncolytic adenovirus.ACTA PHARMACOLOGICA SINICA,28(12),1996-2004.
MLA	Pan, QW,et al."Enhanced sensitivity of hepatocellular carcinoma cells to chemotherapy with a Smac-armed oncolytic adenovirus".ACTA PHARMACOLOGICA SINICA 28.12(2007):1996-2004.