Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1

	Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1
	Jian-Ke, R; Jia-Juan, S; Zhe-Jin, S; Jian, F
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2008
卷号	35 期号:12 页码:1410-1416
关键词	specificity pancreatic lipase related protein 1 (PLRP1) polyclonal antibody GST fusion protein
通讯作者	Jian, F (reprint author), Shanghai Nan Fang Model Orgnasim Res Ctr, Shanghai 201203, Peoples R China.,jfei@mail.tongji.edu.cn
英文摘要	Pancreatic acinar cells synthesize pancreatic lipase related protein 1 (PLRP1), which has a high degree of sequence and structural homology with pancreatic triglyceride lipase (PTL). PTL is required for efficient dietary triglyceride digestion, while the physiological role of PLRP1 has not been elucidated, although some investigations have shown that its expression level is changed under some physiological or pathological conditions. Specific antigenic peptides were fused to glutathione S-transferase (GST) and purified recombinant fusion protein was used to generate polyclonal antisera by immunization of rabbits. The antisera could detect the antigen as low as 0.6 ng and PLRP1 protein in 3 mu g of mouse pancreatic juice extracts. The high specificity was verified in Western blot and immunohistochemistry analysis by using PLRP1 knockout (KO) mice as the control. Furthermore, it was showed that food intake could increase the exocrine secretion of PLRP1 into pancreatic juice. This implied that PLRP1 may fulfill dietary digestion function in the digestion track.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	KINETIC-PROPERTIES ; MESSENGER-RNA ; IDENTIFICATION ; COLIPASE ; GENE ; GP-3 ; MEMBRANE ; CLONING ; MOUSE ; JUICE
收录类别	SCI
语种	中文
WOS记录号	WOS:000262041400010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1461]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jian-Ke, R,Jia-Juan, S,Zhe-Jin, S,et al. Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2008,35(12):1410-1416.
APA	Jian-Ke, R,Jia-Juan, S,Zhe-Jin, S,&Jian, F.(2008).Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,35(12),1410-1416.
MLA	Jian-Ke, R,et al."Preparation, Characterization and Application of High Specific Polyclonal Antibody Against Pancreatic Lipase Related Protein 1".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 35.12(2008):1410-1416.