Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis

	Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis
	Yang, S; Liu, X; Yin, YQ; Fukuda, MN; Zhou, JW
刊名	FASEB JOURNAL
	2008
卷号	22 期号:6 页码:1960-1972
关键词	microtubules mitotic block
通讯作者	Zhou, JW (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jwzhou@ion.ac.cn
英文摘要	Tastin was previously characterized as an accessory protein for cell adhesion that participates in early embryo implantation. Here, we report that tastin is also required for spindle assembly during mitosis. Tastin protein levels peaked in the G(2)/M phase and abruptly declined after cell division. Microscopy showed that tastin is primarily localized on the microtubules, centrosomes, and the mitotic spindle during the cell cycle. Tastin interacted with the dynein intermediate chain, p150(Glued), and gamma-tubulin in addition to Tctex-1 ( the light chain of dynein). Overexpression of tastin led to monopolar spindle formation, whereas loss of tastin expression caused profound mitotic block and preferentially induced multipolar spindles. These multipolar spindles were generated through a loss of cohesion in mitotic centrosomes; specifically, tastin depletion caused the fragmentation of pericentrosomal material and the splitting of the centrioles at the spindle poles. Tastin depletion induced centrosome abnormalities exclusively during mitosis and required both microtubule integrity and Eg5 activity. However, tastin depletion did not disrupt the organization of spindle poles, as revealed by localization of nuclear mitotic apparatus protein (NuMA) and the p150(Glued) component of dynactin. These data indicate that the major function of tastin during mitosis is to maintain the structural and dynamic features of centrosomes, thereby contributing to spindle bipolarity.
学科主题	Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biology ; Cell Biology
关键词[WOS]	ENDOMETRIAL EPITHELIAL-CELLS ; MITOTIC SPINDLE ; CYTOPLASMIC DYNEIN ; LIGHT-CHAIN ; PROTEIN ; COMPLEX ; ORGANIZATION ; MECHANISMS ; TROPHININ ; INTERACTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000256352700035
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1393]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, S,Liu, X,Yin, YQ,et al. Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis[J]. FASEB JOURNAL,2008,22(6):1960-1972.
APA	Yang, S,Liu, X,Yin, YQ,Fukuda, MN,&Zhou, JW.(2008).Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis.FASEB JOURNAL,22(6),1960-1972.
MLA	Yang, S,et al."Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis".FASEB JOURNAL 22.6(2008):1960-1972.