CpG methylation plays a vital role in determining tissue- and cell-specific expression of the human cell-death-inducing DFF45-like effector A gene through the regulation of Sp1/Sp3 binding

	CpG methylation plays a vital role in determining tissue- and cell-specific expression of the human cell-death-inducing DFF45-like effector A gene through the regulation of Sp1/Sp3 binding
	Li, D; Da, L; Tang, H; Li, T; Zhao, M
刊名	NUCLEIC ACIDS RESEARCH
	2008
卷号	36 期号:1 页码:330-341
通讯作者	Zhao, M (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn
英文摘要	Cell-death-inducing DFF45-like effector A (CIDE-A) belongs to a family of proapoptotic proteins, the expression of which is highly restricted in human tissues and cells. Here, the core region of the human CIDE-A promoter was characterized. Surprisingly, two Sp1/Sp3-binding sites, rather than tissue-specific transcription factors, were found to be required for the promoter activity. Although the ubiquitously expressed Sp1 and Sp3 were crucial, they alone could not adequately regulate the specific expression of CIDE-A. We found that the expression of CIDE-A was further regulated by CpG methylation of the promoter region. By performing bisulfite sequencing, we observed dense CpG methylation of the promoter region in tissues and cells with low or no expression of CIDE-A but not in tissues with high level of CIDE-A expression. In vitro methylation of this region showed significantly reduced transcriptional activity. Treatment of CIDE-A-negative cells with 5-aza-2'-deoxycytidine demethylated the CpG sites; this opened the closed chromatin conformation and markedly enhanced the binding affinity of Sp1/Sp3 to the promoter in vivo, thereby restoring CIDE-A expression. These data indicated that CpG methylation plays a crucial role in establishing and maintaining tissue- and cell-specific transcription of the CIDE-A gene through the regulation of Sp1/Sp3 binding.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	DNA-FRAGMENTATION-FACTOR ; TRANSCRIPTION FACTOR ; HISTONE MODIFICATIONS ; ADIPOSE-TISSUE ; CANCER-CELLS ; LUNG-CANCER ; PROMOTER ; PROTEIN ; CHROMATIN ; OBESITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000252545100039
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1327]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, D,Da, L,Tang, H,et al. CpG methylation plays a vital role in determining tissue- and cell-specific expression of the human cell-death-inducing DFF45-like effector A gene through the regulation of Sp1/Sp3 binding[J]. NUCLEIC ACIDS RESEARCH,2008,36(1):330-341.
APA	Li, D,Da, L,Tang, H,Li, T,&Zhao, M.(2008).CpG methylation plays a vital role in determining tissue- and cell-specific expression of the human cell-death-inducing DFF45-like effector A gene through the regulation of Sp1/Sp3 binding.NUCLEIC ACIDS RESEARCH,36(1),330-341.
MLA	Li, D,et al."CpG methylation plays a vital role in determining tissue- and cell-specific expression of the human cell-death-inducing DFF45-like effector A gene through the regulation of Sp1/Sp3 binding".NUCLEIC ACIDS RESEARCH 36.1(2008):330-341.