Caulis Sinomenii Extracts Activate DA/NE Transporter and Inhibit 5HT Transporter

	Caulis Sinomenii Extracts Activate DA/NE Transporter and Inhibit 5HT Transporter
	Zhao, G; Bi, C; Qin, GW; Guo, LH
刊名	EXPERIMENTAL BIOLOGY AND MEDICINE
	2009
卷号	234 期号:8 页码:976-985
关键词	Caulis Sinomenii dopamine norepinephrine serotonin reuptake modulator transporter
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lhguo@sibs.ac.cn
英文摘要	Caulis Sinomenii (QFT) has analgesic, sedative, and anxiolytic-like actions, and is proven effective for improving drug dependence that is known to be associated with abnormal monoaminergic transmission. We assessed whether QFT would be biologically active in functionally regulating monoamine transporters using CHO cells expressing dopamine transporter (DAT), norepinephrine transporter (NET), or serotonin transporter (SERT) (i.e. D8, N1, or S6 cells, respectively). Here, we showed that its primary extracts, such as QA, QC, QE, QD, and QB (QFT ethanol, chloroform, ethyl acetate, alkaloid-free chloroform, and alkaloid-containing chloroform extract, respectively), and secondary extracts, such as QE-2, -3, -5, -7, QD-1, -2, -3, -4, -5, and QB-1, -2, -3, -4, -5 (fractioned from QE, QD, and QB, respectively), in differing degrees, either increased DA/NE uptake by corresponding D8/N1 cells or decreased 5HT uptake by S6 cells; wherein, QE-2, QD-3, and QE-7 were potent DAME uptake activators while both QE-7 and QB-5 were potent 5HT uptake inhibitors. Furthermore, the enhancement of DA/NE uptake was dependent of DAT/NET activity, and the inhibition of 5HT uptake was typical of competition. Thus, QFT extracts, especially QE-2 and QE-7 (both with stronger potencies), are novel monoamine transporter modulators functioning as DAT/NET activators and/or SERT inhibitors, and would likely improve neuropsychological disorders through regulating monoamine transporters. Exp Biol Med 234:976-985, 2009
学科主题	Research & Experimental Medicine
类目[WOS]	Medicine, Research & Experimental
关键词[WOS]	MONOAMINE TRANSPORTERS ; DOPAMINE TRANSPORTER ; SEROTONIN TRANSPORTER ; RAT MODEL ; MECHANISMS ; ADDICTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000268567300012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1226]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, G,Bi, C,Qin, GW,et al. Caulis Sinomenii Extracts Activate DA/NE Transporter and Inhibit 5HT Transporter[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2009,234(8):976-985.
APA	Zhao, G,Bi, C,Qin, GW,&Guo, LH.(2009).Caulis Sinomenii Extracts Activate DA/NE Transporter and Inhibit 5HT Transporter.EXPERIMENTAL BIOLOGY AND MEDICINE,234(8),976-985.
MLA	Zhao, G,et al."Caulis Sinomenii Extracts Activate DA/NE Transporter and Inhibit 5HT Transporter".EXPERIMENTAL BIOLOGY AND MEDICINE 234.8(2009):976-985.