Inhibition of renal cancer cell growth by oncolytic adenovirus armed short hairpin RNA targeting hTERT gene

	Inhibition of renal cancer cell growth by oncolytic adenovirus armed short hairpin RNA targeting hTERT gene
	Zheng, JN; Pei, DS; Sun, FH; Zhang, BF; Liu, XY; Gu, JF; Liu, YH; Hu, XL; Mao, LJ; Wen, RM
刊名	CANCER BIOLOGY & THERAPY
	2009
卷号	8 期号:1 页码:84-91
关键词	RNAi hTERT oncolytic adenovirus renal cancer shRNAs apoptosis gene therapy
通讯作者	Zheng, JN (reprint author), Affiliated Hosp, Lab Urol, Xuzhou Med Coll, 99 W Huai Hai Rd, Xuzhou, Jiangsu, Peoples R China.,jnzheng@xzmc.edu.cn
英文摘要	RNA interference (RNAi) has been proved to be a powerful tool for gene knockdown purpose and holds a great promise for the treatment of cancer. Our previous study demonstrated that the reduction of hTERT expression by means of chemically synthesized siRNAs and shRNAs expressed from plasmid resulted in proliferation inhibition in human renal carcinoma cells. In this study, we constructed a novel oncolytic adenovirus-based shRNA expression system, ZD55-hTERT and to explore ZD55-hTERT-mediated RNAi for hTERT gene silencing. Our results showed that ZD55-hTERT could induce silencing of hTERT gene effectively, allow for efficient tumor-specific viral replication and induce the apoptosis of tumor cells effectively in vitro and in nude mice. We conclude that combining shRNA gene therapy and oncolytic virotherapy can enhance antitumor efficacy as a result of synergism between CRAd oncolysis and shRNA antitumor responses.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	TELOMERASE ACTIVITY ; BLADDER-CANCER ; NECK-CANCER ; EXPRESSION ; ONYX-015 ; PROLIFERATION ; INTERFERENCE ; REPLICATION ; COMBINATION ; EFFICACY
收录类别	SCI
语种	英语
WOS记录号	WOS:000264207500019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1127]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zheng, JN,Pei, DS,Sun, FH,et al. Inhibition of renal cancer cell growth by oncolytic adenovirus armed short hairpin RNA targeting hTERT gene[J]. CANCER BIOLOGY & THERAPY,2009,8(1):84-91.
APA	Zheng, JN.,Pei, DS.,Sun, FH.,Zhang, BF.,Liu, XY.,...&Li, W.(2009).Inhibition of renal cancer cell growth by oncolytic adenovirus armed short hairpin RNA targeting hTERT gene.CANCER BIOLOGY & THERAPY,8(1),84-91.
MLA	Zheng, JN,et al."Inhibition of renal cancer cell growth by oncolytic adenovirus armed short hairpin RNA targeting hTERT gene".CANCER BIOLOGY & THERAPY 8.1(2009):84-91.