Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine

	Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine
	Zhao, G; Wang, J; Qin, GW; Guo, LH
刊名	NEUROCHEMICAL RESEARCH
	2010
卷号	35 期号:4 页码:666-676
关键词	Cynomorium songaricum Rupr gamma-Aminobutyric acid Dopamine Norepinephrine Serotonin Transporter modulator
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lhguo@sibs.ac.cn
英文摘要	Cynomorium songaricum Rupr. (SY) is a central nervous system-oriented herb material that has actions of anti-dementia, anti-epilepsy, and anti-stress. It is unclear whether SY would be biologically active in functionally regulating neurotransmitter transporters. Here, we assessed these potential actions using Chinese hamster ovary cells expressing gamma-aminobutyric acid (GABA) transporter (GAT-1), dopamine transporter (DAT), norepinephrine transporter (NET), or serotonin transporter (SERT) (i.e. G1, D8, N1, or S6 cells, respectively). It was shown that SY extracts, such as SYw, SYa, SYp, SYc, SYe, and SYb (SY water, ethanol, petroleum ether, chloroform, ethyl acetate, and n-butyl alcohol extract, respectively) increased dopamine/norepinephrine (DA/NE) uptake by corresponding D8/N1 cells and decreased gamma-aminobutyric acid/serotoin (GABA/5HT) uptake by corresponding G1/S6 cells; wherein, the potency or efficacy of SYc for up-regulating DA/NE uptake and that of SYb for inhibiting GABA/5HT uptake were relatively stronger. Additionally, GABA/5HT-uptake inhibition by SY extracts were also seen in cortical synaptosomes, and DA/NE-uptake enhancement by SYc was dependent on the activity of DAT and NET. Thus, SY extracts especially SYc and SYb are novel neurotransmitter-transporter modulators functioning as DAT/NET activators and/or GAT-1/SERT inhibitors.
学科主题	Biochemistry & Molecular Biology; Neurosciences & Neurology
类目[WOS]	Biochemistry & Molecular Biology ; Neurosciences
关键词[WOS]	DOPAMINE TRANSPORTER ; SEROTONIN TRANSPORTER ; NORADRENALINE TRANSPORTER ; GABA TRANSPORTER ; CONSTITUENTS ; INHIBITION ; MECHANISMS ; ADDICTION ; STEMS ; L.
收录类别	SCI
语种	英语
WOS记录号	WOS:000275820500020
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/995]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, G,Wang, J,Qin, GW,et al. Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine[J]. NEUROCHEMICAL RESEARCH,2010,35(4):666-676.
APA	Zhao, G,Wang, J,Qin, GW,&Guo, LH.(2010).Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine.NEUROCHEMICAL RESEARCH,35(4),666-676.
MLA	Zhao, G,et al."Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine".NEUROCHEMICAL RESEARCH 35.4(2010):666-676.