The protective effects of alpha-ketoacids against oxidative stress on rat spermatozoa in vitro

	The protective effects of alpha-ketoacids against oxidative stress on rat spermatozoa in vitro
	Li, SF; Liu, HX; Zhang, YB; Yan, YC; Li, YP
刊名	ASIAN JOURNAL OF ANDROLOGY
	2010
卷号	12 期号:2 页码:247-256
关键词	alpha-ketoacids antioxidants oxidative stress reactive oxygen species
通讯作者	Li, YP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,Lab Mol Cell Biol, Shanghai 200031, Peoples R China.,Yipingli@sibs.ac.cn
英文摘要	The aim of this study was to determine the effects of antioxidants, including alpha-ketoacids (alpha-ketoglutarate and pyruvate), lactate and glutamate/malate combination, against oxidative stress on rat spermatozoa. Our results showed that H(2)O(2) (250 mmol L(-1))-induced damages, such as impaired motility, adenosine triphosphate (ATP) depletion, inhibition of sperm protein phosphorylation, reduced acrosome reaction and decreased viability, could be significantly prevented by incubation of the spermatozoa with a-ketoglutarate (4 mmol L(-1)) or pyruvate (4 mmol L-1). Without exogenous H(2)O(2) in the medium, the addition of pyruvate (4 mmol L(-1)) significantly increased the superoxide anion (O2(-center dot)) level in sperm suspension (P <= 0.01), whereas the addition of a-ketoglutarate (4 mmol L(-1)) and lactate (4 mmol L(-1)) significantly enhanced tyrosine-phosphorylated proteins with the size of 95 kDa (P <= 0.04). At the same time, alpha-ketoglutarate, pyruvate, lactate, glutamate and malate supplemented in media can be used as important energy sources and supply ATP for sperm motility. In conclusion, the present results show that a-ketoacids could be effective antioxidants for protecting rat spermatozoa from H(2)O(2) attack and could be effective components to improve the antioxidant capacity of Biggers, Whitten and Whittingham media.
学科主题	Endocrinology & Metabolism; Urology & Nephrology
类目[WOS]	Andrology ; Urology & Nephrology
关键词[WOS]	SPERM CAPACITATION ; ACROSOME REACTION ; HYDROGEN-PEROXIDE ; SEMEN QUALITY ; ANTIOXIDANT SUPPLEMENTATION ; PHOSPHORYLATION EVENTS ; LIPID-PEROXIDATION ; EQUINE SPERMATOZOA ; FERTILITY ; MOTILITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000275109700016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/952]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, SF,Liu, HX,Zhang, YB,et al. The protective effects of alpha-ketoacids against oxidative stress on rat spermatozoa in vitro[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(2):247-256.
APA	Li, SF,Liu, HX,Zhang, YB,Yan, YC,&Li, YP.(2010).The protective effects of alpha-ketoacids against oxidative stress on rat spermatozoa in vitro.ASIAN JOURNAL OF ANDROLOGY,12(2),247-256.
MLA	Li, SF,et al."The protective effects of alpha-ketoacids against oxidative stress on rat spermatozoa in vitro".ASIAN JOURNAL OF ANDROLOGY 12.2(2010):247-256.