An armed oncolytic adenovirus ZD55-IL-24 combined with ADM or DDP demonstrated enhanced antitumor effect in lung cancer

	An armed oncolytic adenovirus ZD55-IL-24 combined with ADM or DDP demonstrated enhanced antitumor effect in lung cancer
	Zhong, SY; Yu, DB; Wang, YG; Qiu, SB; Wu, SJ; Liu, XY
刊名	ACTA ONCOLOGICA
	2010
卷号	49 期号:1 页码:91-99
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	Purpose. Oncolytic adenovirus such as ZD55 has become a promising anticancer agent for its efficient tumor-targeted replication and lysis capability. Armed with therapeutic gene IL-24 to generate a novel oncolytic adenovirus ZD55-IL-24, the antitumor efficiency of ZD55 is greatly increased. To explore the clinical application of ZD55-IL-24 in cancer therapy, the combination of gene-virotherapy (ZD55-IL-24) with chemotherapy was performed in this paper. Methods. The effect of this gene-virotherapy with chemotherapy on cell proliferation was determined by MTT assay in four types of cancer cell lines and one human normal cell line. Real-time PCR was performed to detect the replication of ZD55-IL-24 when adriamycin (ADM) or cisplatin (DDP) was administrated. The changes in caspase pathway were analyzed by Western blot. We further identify the combinational therapy in Balb/c nude mice with NCI-H460 xenograft. Results. ADM and DDP enhanced cell killing/inhibiting effects of ZD55-IL-24 in all the tumor cell lines, while no overlapping toxicity was observed in the normal liver cell line L-02. These chemo-agents inhibited the propagation of ZD55-IL-24 in NCI-H460 cells, but did not influence the expression of IL-24. Consistent with the results in vitro, the tumor growth of co-administration group was remarkably delayed, compared with single treatment groups (p < 0.05). Conclusion. ZD55-IL-24 combined with ADM demonstrates improved killing effects against lung tumor xenograft.
学科主题	Oncology
类目[WOS]	Oncology
收录类别	SCI
语种	英语
WOS记录号	WOS:000273953700012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/923]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhong, SY,Yu, DB,Wang, YG,et al. An armed oncolytic adenovirus ZD55-IL-24 combined with ADM or DDP demonstrated enhanced antitumor effect in lung cancer[J]. ACTA ONCOLOGICA,2010,49(1):91-99.
APA	Zhong, SY,Yu, DB,Wang, YG,Qiu, SB,Wu, SJ,&Liu, XY.(2010).An armed oncolytic adenovirus ZD55-IL-24 combined with ADM or DDP demonstrated enhanced antitumor effect in lung cancer.ACTA ONCOLOGICA,49(1),91-99.
MLA	Zhong, SY,et al."An armed oncolytic adenovirus ZD55-IL-24 combined with ADM or DDP demonstrated enhanced antitumor effect in lung cancer".ACTA ONCOLOGICA 49.1(2010):91-99.