Combined Activin A/LiCl/Noggin Treatment Improves Production of Mouse Embryonic Stem Cell-Derived Definitive Endoderm Cells

	Combined Activin A/LiCl/Noggin Treatment Improves Production of Mouse Embryonic Stem Cell-Derived Definitive Endoderm Cells
	Li, FM; He, ZY; Li, YF; Liu, PY; Chen, F; Wang, MJ; Zhu, HY; Ding, XY; Wangensteen, KJ; Hu, YP
刊名	JOURNAL OF CELLULAR BIOCHEMISTRY
	2011
卷号	112 期号:4 页码:1022-1034
关键词	MOUSE EMBRYONIC STEM CELLS DEFINITIVE ENDODERM SUSPENSION CULTURE EMBRYONIC BODY DIFFERENTIATION
通讯作者	Wang, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Lab Mol & Cell Biol, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,wangx@sibs.ac.cn
英文摘要	Induction of definitive endoderm (DE) cells is a prerequisite for the whole process of embryonic stem (ES) cells differentiating into hepatic or pancreatic progenitor cells. We have established an efficient method to induce mouse ES cell-derived DE cells in suspension embryonic body (EB) culture. Similar to previous studies, mouse ES cell-derived DE cells, which were defined as Cxcr4(+)c-Kit(+),Cxcr4(+)E-cadherin(+) cells or Cxcr4(+)PDGFRa(-) cells, could be induced in the serum-free EBs at Day 4 of induction. The activations of Wnt, Nodal, and FGF signaling pathways in differentiating EBs promoted DE cell differentiation, while activation of BMP4 signaling inhibited the process. In the present study, we found that chemical activation of canonical Wnt signaling pathway by LiCl could synergize with Activin A-mediated Nodal signaling pathway to promote induction of DE cells, and inhibition of Bmp4 signaling by Noggin along with Activin A/LiCl further improved the efficiency of DE cell differentiation. The derived DE cells were proved for their capacities to become hepatic progenitor cells or pancreatic progenitor cells. In conclusion, we significantly improved the efficiency of generating mouse ES cell-derived DE cells by combined Activin A/LiCl/Noggin treatment. Our work will be greatly helpful to generate ES cell-derived hepatic cells and ES cell-derived pancreatic cells for future regenerative medicine. J. Cell. Biochem. 112: 1022-1034, 2011. (C) 2010 Wiley-Liss, Inc.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	IN-VITRO ; VISCERAL ENDODERM ; LIVER DEVELOPMENT ; WNT/BETA-CATENIN ; PRIMITIVE STREAK ; PROGENITOR CELLS ; SMALL-MOLECULE ; DIFFERENTIATION ; HEPATOCYTES ; WNT
收录类别	SCI
语种	英语
WOS记录号	WOS:000288394000005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/784]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, FM,He, ZY,Li, YF,et al. Combined Activin A/LiCl/Noggin Treatment Improves Production of Mouse Embryonic Stem Cell-Derived Definitive Endoderm Cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2011,112(4):1022-1034.
APA	Li, FM.,He, ZY.,Li, YF.,Liu, PY.,Chen, F.,...&Wang, X.(2011).Combined Activin A/LiCl/Noggin Treatment Improves Production of Mouse Embryonic Stem Cell-Derived Definitive Endoderm Cells.JOURNAL OF CELLULAR BIOCHEMISTRY,112(4),1022-1034.
MLA	Li, FM,et al."Combined Activin A/LiCl/Noggin Treatment Improves Production of Mouse Embryonic Stem Cell-Derived Definitive Endoderm Cells".JOURNAL OF CELLULAR BIOCHEMISTRY 112.4(2011):1022-1034.