Reprogramming of mouse and human somatic cells by high-performance engineered factors

	Reprogramming of mouse and human somatic cells by high-performance engineered factors
	Wang, Y; Chen, JK; Hu, JL; Wei, XX; Qin, DJ; Gao, JA; Zhang, L; Jiang, J; Li, JS; Liu, J
刊名	EMBO REPORTS
	2011
卷号	12 期号:4 页码:373-378
关键词	reprogramming factor iPS induced pluripotency reprogramming stem cells
通讯作者	Pei, DQ (reprint author), Chinese Acad Sci, Guangzhou Inst Biomed, S China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou 510663, Guangdong, Peoples R China.,pei_duanqing@gibh.ac.cn ; glxu@sibs.ac.cn
英文摘要	Reprogramming somatic cells to become induced pluripotent stem cells (iPSCs) by using defined factors represents an important breakthrough in biology and medicine, yet remains inefficient and poorly understood. We therefore devised synthetic factors by fusing the VP16 transactivation domain to OCT4 (also known as Pou5f1), NANOG and SOX2, respectively. These synthetic factors could reprogramme both mouse and human fibroblasts with enhanced efficiency and accelerated kinetics. Remarkably, Oct4-VP16 alone could efficiently reprogramme mouse embryonic fibroblasts (MEFs) into germline-competent iPSCs. Furthermore, episomally delivered synthetic factors could reproducibly generate integration-free iPSCs from MEFs with enhanced efficiency. Our results not only demonstrate the feasibility of engineering more potent reprogramming factors, but also suggest that transcriptional reactivation of OCT4 target genes might be a rate-limiting step in the conversion of somatic cells to pluripotent cells. Synthetic factor-based reprogramming might lead to a paradigm shift in reprogramming research.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	PLURIPOTENT STEM-CELLS ; ACTIVATION DOMAIN ; IPS CELLS ; GENERATION ; TRANSCRIPTION ; VP16 ; FIBROBLASTS ; INDUCTION ; PATHWAY ; LINKING
收录类别	SCI
语种	英语
WOS记录号	WOS:000289073200018
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/782]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, Y,Chen, JK,Hu, JL,et al. Reprogramming of mouse and human somatic cells by high-performance engineered factors[J]. EMBO REPORTS,2011,12(4):373-378.
APA	Wang, Y.,Chen, JK.,Hu, JL.,Wei, XX.,Qin, DJ.,...&Xu, GL.(2011).Reprogramming of mouse and human somatic cells by high-performance engineered factors.EMBO REPORTS,12(4),373-378.
MLA	Wang, Y,et al."Reprogramming of mouse and human somatic cells by high-performance engineered factors".EMBO REPORTS 12.4(2011):373-378.