Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway

	Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway
	Xie, J; Jiang, H; Wan, YH; Du, AY; Guo, KJ; Liu, T; Ye, WY; Niu, X; Wu, J; Dong, XQ
刊名	JOURNAL OF MOLECULAR CELL BIOLOGY
	2011
卷号	3 期号:4 页码:250-259
关键词	acetylcholinesterase apoptosis 55 kDa AChE caspase PI3K/Akt pathway
通讯作者	Zhang, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 YueYang Rd, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.cn
英文摘要	Acetylcholinesterase (AChE) is emerging as an important contributor to apoptosis in various cell types. However, overexpression of AChE does not initiate apoptosis, and cells which express AChE at basal levels grow normally, suggesting that AChE may function differently between normal and apoptotic conditions. In this study, we determined that an AChE-derived protein (similar to 55 kDa) positively correlated with cellular apoptotic levels. The 55 kDa AChE protein was not a result of a novel splice variant of the AChE primary transcript. Instead, it was determined to be a cleaved fragment of the full-length 68 kDa AChE protein that could not be inhibited by cycloheximide (CHX) but could be suppressed by caspase inhibitors in apoptotic PC-12 cells. Furthermore, activation of the Akt cascade abolished the 55 kDa protein, and both AChE protein forms (68 and 55 kDa) accumulated in the nucleus during apoptosis. In a mouse model for ischemia/reperfusion (I/R)-induced acute renal failure, the 55 kDa AChE protein was detected in the impaired organs but not in the normal ones, and its levels correlated with the genotype of the mice. In summary, a 55 kDa AChE protein resulting from the cleavage of 68 kDa AChE is induced during apoptosis, and it is negatively regulated by the Akt pathway. This study suggests that an alternative form of AChE may play a role in apoptosis.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	NONNEURONAL CHOLINERGIC SYSTEM ; NEURONAL CELL-DEATH ; SURVIVAL SIGNALS ; EXPRESSION ; CANCER ; ACHE ; PHOSPHORYLATION ; CHOLINESTERASES ; INHIBITION ; NEUROLIGIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000293624000007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/771]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xie, J,Jiang, H,Wan, YH,et al. Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2011,3(4):250-259.
APA	Xie, J.,Jiang, H.,Wan, YH.,Du, AY.,Guo, KJ.,...&Zhang, XJ.(2011).Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway.JOURNAL OF MOLECULAR CELL BIOLOGY,3(4),250-259.
MLA	Xie, J,et al."Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway".JOURNAL OF MOLECULAR CELL BIOLOGY 3.4(2011):250-259.