Large Scale Phosphoproteome Profiles Comprehensive Features of Mouse Embryonic Stem Cells

	Large Scale Phosphoproteome Profiles Comprehensive Features of Mouse Embryonic Stem Cells
	Li, QR; Xing, XB; Chen, TT; Li, RX; Dai, J; Sheng, QH; Xin, SM; Zhu, LL; Jin, Y; Pei, G
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2011
卷号	10 期号:4 页码:1750-1750
通讯作者	Kang, JH (reprint author), Chinese Acad Sci, Key Lab Syst Biol, Shanghai 200031, Peoples R China.,jhkang@sibs.ac.cn ; yxli@sibs.ac.cn ; zr@sibs.ac.cn
英文摘要	Embryonic stem cells are pluripotent and capable of unlimited self-renewal. Elucidation of the underlying molecular mechanism may contribute to the advancement of cell-based regenerative medicine. In the present work, we performed a large scale analysis of the phosphoproteome in mouse embryonic stem (mES) cells. Using multiplex strategies, we detected 4581 proteins and 3970 high confidence distinct phosphosites in 1642 phosphoproteins. Notably, 22 prominent phosphorylated stem cell marker proteins with 39 novel phosphosites were identified for the first time by mass spectrometry, including phosphorylation sites in NANOG (Ser-65) and RE1 silencing transcription factor (Ser-950 and Thr-953). Quantitative profiles of NANOG peptides obtained during the differentiation of mES cells revealed that the abundance of phosphopeptides and non-phosphopeptides decreased with different trends. To our knowledge, this study presents the largest global characterization of phosphorylation in mES cells. Compared with a study of ultimately differentiated tissue cells, a bioinformatics analysis of the phosphorylation data set revealed a consistent phosphorylation motif in human and mouse ES cells. Moreover, investigations into phosphorylation conservation suggested that phosphoproteins were more conserved in the undifferentiated ES cell state than in the ultimately differentiated tissue cell state. However, the opposite conclusion was drawn from this conservation comparison with phosphosites. Overall, this work provides an overview of phosphorylation in mES cells and is a valuable resource for the future understanding of basic biology in mES cells. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.001750, 1-14, 2011.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	SELF-RENEWAL ; MASS-SPECTROMETRY ; ES CELLS ; PHOSPHORYLATION DYNAMICS ; PROTEIN-PHOSPHORYLATION ; PROTEOMIC ANALYSIS ; CULTURE SILAC ; CONTINUOUS PH ; AMINO-ACIDS ; GERM-CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000289067300001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/768]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, QR,Xing, XB,Chen, TT,et al. Large Scale Phosphoproteome Profiles Comprehensive Features of Mouse Embryonic Stem Cells[J]. MOLECULAR & CELLULAR PROTEOMICS,2011,10(4):1750-1750.
APA	Li, QR.,Xing, XB.,Chen, TT.,Li, RX.,Dai, J.,...&Zeng, R.(2011).Large Scale Phosphoproteome Profiles Comprehensive Features of Mouse Embryonic Stem Cells.MOLECULAR & CELLULAR PROTEOMICS,10(4),1750-1750.
MLA	Li, QR,et al."Large Scale Phosphoproteome Profiles Comprehensive Features of Mouse Embryonic Stem Cells".MOLECULAR & CELLULAR PROTEOMICS 10.4(2011):1750-1750.