Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain

	Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain
	Wang, S; Du, JM; Tang, H; Ding, XY; Zha, MW; Xu, ZF
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2011
卷号	43 期号:2 页码:149-153
关键词	crystallization glutamine LEF1 Notch selenomethionine TLE1 X-ray prokaryotic expression
通讯作者	Zha, MW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Res Ctr Struct Biol, Shanghai 200031, Peoples R China.,mwzha@sibs.ac.cn ; xu_zhi_fei@yahoo.com.cn
英文摘要	Human transducin-like enhancer of split 1 (TLE1) plays crucial roles in a number of developmental processes and is involved in pathogenesis of malignancy tumors. The N-terminal glutamine-rich domain (Q domain) of TLE1 mediates its tetramerization and interactions with different DNA-binding transcription factors to regulate Notch and Wnt signaling pathways. To better understand the molecular mechanism of TLE1's functions in these pathways, we cloned, purified, and crystallized the TLE1 Q domain (TLE1-Q). The crystals belong to space group C222(1), with the complete diffraction data of the native and Se-Met TLE1-Q collected to 3.5 and 4.1 angstrom resolutions, respectively. The phasing-solving and model building are in progress.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	TRANSCRIPTIONAL REPRESSION ; BETA-CATENIN ; GROUCHO ; PROTEINS ; COREPRESSORS ; INTERACT ; FAMILY ; MODEL ; ACTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000286670900009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/747]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, S,Du, JM,Tang, H,et al. Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(2):149-153.
APA	Wang, S,Du, JM,Tang, H,Ding, XY,Zha, MW,&Xu, ZF.(2011).Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(2),149-153.
MLA	Wang, S,et al."Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.2(2011):149-153.