Transcriptome analysis of microRNAs in developing cerebral cortex of rat

	Transcriptome analysis of microRNAs in developing cerebral cortex of rat
	Yao, MJ; Chen, G; Zhao, PP; Lu, MH; Jian, J; Liu, MF; Yuan, XB
刊名	BMC GENOMICS
	2012
卷号	13 期号:1 页码:232-232
关键词	MicroRNA RNA editing Cerebral cortex Development
通讯作者	Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China.,yuanxb@ion.ac.cn
英文摘要	Background: The morphogenesis of the cerebral cortex depends on the precise control of gene expression during development. Small non-coding RNAs, including microRNAs and other groups of small RNAs, play profound roles in various physiological and pathological processes via their regulation of gene expression. A systematic analysis of the expression profile of small non-coding RNAs in developing cortical tissues is important for clarifying the gene regulation networks mediating key developmental events during cortical morphogenesis. Results: Global profiling of the small RNA transcriptome was carried out in rat cerebral cortex from E10 till P28 using next-generation sequencing technique. We found an extraordinary degree of developmental stage-specific expression of a large group of microRNAs. A group of novel microRNAs with functional hints were identified, and brain-enriched expression and Dicer-dependent production of high-abundant novel microRNAs were validated. Profound editing of known microRNAs at "seed" sequence and flanking sequence was observed, with much higher editing events detected at late postnatal stages than embryonic stages, suggesting the necessity of microRNA editing for the fine tuning of gene expression during the formation of complicated synaptic connections at postnatal stages. Conclusion: Our analysis reveals extensive regulation of microRNAs during cortical development. The dataset described here will be a valuable resource for clarifying new regulatory mechanisms for cortical development and diseases and will greatly contribute to our understanding of the divergence, modification, and function of microRNAs.
学科主题	Biotechnology & Applied Microbiology; Genetics & Heredity
类目[WOS]	Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]	EMBRYONIC STEM-CELLS ; SMALL NONCODING RNAS ; OLIGODENDROCYTE DIFFERENTIATION ; L1 RETROTRANSPOSITION ; BRAIN-DEVELOPMENT ; HUMAN FIBROBLASTS ; SEED REGION ; EXPRESSION ; DROSOPHILA ; NEURONS
收录类别	SCI
语种	英语
WOS记录号	WOS:000308932900002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/701]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yao, MJ,Chen, G,Zhao, PP,et al. Transcriptome analysis of microRNAs in developing cerebral cortex of rat[J]. BMC GENOMICS,2012,13(1):232-232.
APA	Yao, MJ.,Chen, G.,Zhao, PP.,Lu, MH.,Jian, J.,...&Yuan, XB.(2012).Transcriptome analysis of microRNAs in developing cerebral cortex of rat.BMC GENOMICS,13(1),232-232.
MLA	Yao, MJ,et al."Transcriptome analysis of microRNAs in developing cerebral cortex of rat".BMC GENOMICS 13.1(2012):232-232.