Structure of Sad1-UNC84 Homology (SUN) Domain Defines Features of Molecular Bridge in Nuclear Envelope.

	Structure of Sad1-UNC84 Homology (SUN) Domain Defines Features of Molecular Bridge in Nuclear Envelope.
	Zhou, ZC; Du, XL; Cai, Z; Song, XM; Zhang, HT; Mizuno, T; Suzuki, E; Yee, MR; Berezov, A; Murali, R
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2012
卷号	287 期号:8 页码:5317-5326
通讯作者	Greene, MI (reprint author), Univ Penn, Dept Pathol & Lab Med, Perelman Sch Med, 252 John Morgan Bldg,36th St & Hamilton Walk, Philadelphia, PA 19104 USA.,greene@reo.med.upenn.edu ; qiang.wang@cshs.org
英文摘要	The SUN (Sad1-UNC-84 homology) domain is conserved in a number of nuclear envelope proteins involved in nuclear migration, meiotic telomere tethering, and antiviral responses. The LINC (linker of nucleoskeleton and cytoskeleton) complex, formed by the SUN and the nesprin proteins at the nuclear envelope, serves as a mechanical linkage across the nuclear envelope. Here we report the crystal structure of the SUN2 protein SUN domain, which reveals a homotrimer. The SUN domain is sufficient to mediate binding to the KASH (Klarsicht, ANC-1, and Syne homology) domain of nesprin 2, and the regions involved in the interaction have been identified. Binding of the SUN domain to the KASH domain is abolished by deletion of a region important for trimerization or by point mutations associated with nuclear migration failure. We propose a model of the LINC complex, where the SUN and the KASH domains form a higher ordered oligomeric network in the nuclear envelope. These findings provide the structural basis for understanding the function and the regulation of the LINC complex.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	NEMATODE CAENORHABDITIS-ELEGANS ; SPINDLE POLE BODY ; MEMBRANE PROTEIN ; ACTIN CYTOSKELETON ; EGF RECEPTOR ; MIGRATION ; LOCALIZATION ; INTERACTS ; NESPRINS ; BOUQUET
收录类别	SCI
语种	英语
WOS记录号	WOS:000300638000014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/634]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, ZC,Du, XL,Cai, Z,et al. Structure of Sad1-UNC84 Homology (SUN) Domain Defines Features of Molecular Bridge in Nuclear Envelope.[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(8):5317-5326.
APA	Zhou, ZC.,Du, XL.,Cai, Z.,Song, XM.,Zhang, HT.,...&Wang, Q.(2012).Structure of Sad1-UNC84 Homology (SUN) Domain Defines Features of Molecular Bridge in Nuclear Envelope..JOURNAL OF BIOLOGICAL CHEMISTRY,287(8),5317-5326.
MLA	Zhou, ZC,et al."Structure of Sad1-UNC84 Homology (SUN) Domain Defines Features of Molecular Bridge in Nuclear Envelope.".JOURNAL OF BIOLOGICAL CHEMISTRY 287.8(2012):5317-5326.