miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics

	miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics
	Cao, JL; Shen, YD; Zhu, L; Xu, YA; Zhou, YZ; Wu, ZL; Li, YP; Yan, XM; Zhu, XL
刊名	NATURE CELL BIOLOGY
	2012
卷号	14 期号:7 页码:697-706
通讯作者	Yan, XM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,yanx@sibs.ac.cn ; xlzhu@sibs.ac.cn
英文摘要	Ciliogenesis requires the removal of CP110 from the mother centriole; actin dynamics also influence ciliation, at least partly by affecting the centrosomal accumulation of ciliogenic membrane vesicles. How these distinct processes are properly regulated remains unknown. Here we show that miR-129-3p, a microRNA conserved in vertebrates, controlled cilia biogenesis in cultured cells by concomitantly downregulating CP110 and repressing branched F.-actin formation. Blocking miR-129-3p inhibited serum-starvation-induced ciliogenesis, whereas its overexpression potently induced ciliation in proliferating cells and also promoted cilia elongation. Gene expression analysis further identified ARP2, TOCA1, ABLIM1 and ABLIM3 as its targets in ciliation-related actin dynamics. Moreover, miR-129-3p inhibition in zebrafish embryos suppressed ciliation in Kupffer's vesicle and the pronephros, and induced developmental abnormalities including a curved body, pericardial oedema and defective left right asymmetry. Therefore, our results reveal a mechanism that orchestrates both the centriole-to-basal body transition and subsequent cilia assembly through microRNA-mediated post-transcriptional regulation.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ARP2/3 COMPLEX ; MOLECULAR CHARACTERIZATION ; EPITHELIAL-CELLS ; KUPFFERS VESICLE ; SIGNALING CENTER ; MIGRATING CELLS ; PROTEIN ; CILIOGENESIS ; ZEBRAFISH ; MICRORNAS
收录类别	SCI
语种	英语
WOS记录号	WOS:000305921200009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/621]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cao, JL,Shen, YD,Zhu, L,et al. miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics[J]. NATURE CELL BIOLOGY,2012,14(7):697-706.
APA	Cao, JL.,Shen, YD.,Zhu, L.,Xu, YA.,Zhou, YZ.,...&Zhu, XL.(2012).miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.NATURE CELL BIOLOGY,14(7),697-706.
MLA	Cao, JL,et al."miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics".NATURE CELL BIOLOGY 14.7(2012):697-706.