Prostate Cancer-Specific and Potent Antitumor Effect of a DD3-Controlled Oncolytic Virus Harboring the PTEN Gene

	Prostate Cancer-Specific and Potent Antitumor Effect of a DD3-Controlled Oncolytic Virus Harboring the PTEN Gene
	Ding, M; Cao, X; Xu, HN; Fan, JK; Huang, HL; Yang, DQ; Li, YH; Wang, J; Li, RS; Liu, XY
刊名	PLOS ONE
	2012
卷号	7 期号:4 页码:e35153-e35153
通讯作者	Ding, M (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai, Peoples R China.,runshengli2007@gmail.com ; xyliu@sibs.ac.cn
英文摘要	Prostate cancer is a major health problem for men in Western societies. Here we report a Prostate Cancer-Specific Targeting Gene-Viro-Therapy (CTGVT-PCa), in which PTEN was inserted into a DD3-controlled oncolytic viral vector (OV) to form Ad.DD3.E1A.E1B(Delta 55)-(PTEN) or, briefly, Ad.DD3.D55-PTEN. The woodchuck post-transcriptional element (WPRE) was also introduced at the downstream of the E1A coding sequence, resulting in much higher expression of the E1A gene. DD3 is one of the most prostate cancer-specific genes and has been used as a clinical bio-diagnostic marker. PTEN is frequently inactivated in primary prostate cancers, which is crucial for prostate cancer progression. Therefore, the Ad.DD3.D55-PTEN has prostate cancer specific and potent antitumor effect. The tumor growth rate was almost completely inhibited with the final tumor volume after Ad.DD3.D55-PTEN treatment less than the initial volume at the beginning of Ad.DD3.D55-PTEN treatment, which shows the powerful antitumor effect of Ad.DD3.D55-PTEN on prostate cancer tumor growth. The CTGVT-PCa construct reported here killed all of the prostate cancer cell lines tested, such as DU145, 22RV1 and CL1, but had a reduced or no killing effect on all the non-prostate cancer cell lines tested. The mechanism of action of Ad. DD3.D55-PTEN was due to the induction of apoptosis, as detected by TUNEL assays and flow cytometry. The apoptosis was mediated by mitochondria-dependent and -independent pathways, as determined by caspase assays and mitochondrial membrane potential.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	ADENOVIRUS-MEDIATED TRANSFER ; INHIBITS CELL-GROWTH ; REPLICATING ADENOVIRUS ; GLIOBLASTOMA CELLS ; COLORECTAL-CANCER ; IN-VITRO ; THERAPY ; EXPRESSION ; PROMOTER ; VECTORS
收录类别	SCI
语种	英语
WOS记录号	WOS:000305336600089
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/568]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ding, M,Cao, X,Xu, HN,et al. Prostate Cancer-Specific and Potent Antitumor Effect of a DD3-Controlled Oncolytic Virus Harboring the PTEN Gene[J]. PLOS ONE,2012,7(4):e35153-e35153.
APA	Ding, M.,Cao, X.,Xu, HN.,Fan, JK.,Huang, HL.,...&Liu, XY.(2012).Prostate Cancer-Specific and Potent Antitumor Effect of a DD3-Controlled Oncolytic Virus Harboring the PTEN Gene.PLOS ONE,7(4),e35153-e35153.
MLA	Ding, M,et al."Prostate Cancer-Specific and Potent Antitumor Effect of a DD3-Controlled Oncolytic Virus Harboring the PTEN Gene".PLOS ONE 7.4(2012):e35153-e35153.