Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish

	Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish
	Hu, SN; Yu, H; Zhang, YB; Wu, ZL; Yan, YC; Li, YX; Li, YY; Li, YP
刊名	FEBS LETTERS
	2012
卷号	586 期号:3 页码:222-228
关键词	sox31 MBT Apoptosis
通讯作者	Li, YP (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Shanghai Key Lab Mol Androl, Shanghai Inst Biol Sci,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,Yipingli@sibs.ac.cn
英文摘要	Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microarray analysis of mRNAs collected at the dome stage revealed that the Sb MO impairs activation of a large set of zygotic genes and reduces degradation of maternal mRNA during MBT. Furthermore, an apoptotic response occurs in Sb morphants at about 6 hpf. SoxB1 family genes including sox31 thus play an essential role for early embryos traversing the transitional stage. (C) 2011 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
学科主题	Biochemistry & Molecular Biology; Biophysics; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	MIDBLASTULA TRANSITION ; GENE ; EXPRESSION ; TRANSCRIPTION ; ACTIVATION ; EMBRYOS ; MOUSE ; STAGE
收录类别	SCI
语种	英语
WOS记录号	WOS:000300186900004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/566]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, SN,Yu, H,Zhang, YB,et al. Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish[J]. FEBS LETTERS,2012,586(3):222-228.
APA	Hu, SN.,Yu, H.,Zhang, YB.,Wu, ZL.,Yan, YC.,...&Li, YP.(2012).Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish.FEBS LETTERS,586(3),222-228.
MLA	Hu, SN,et al."Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish".FEBS LETTERS 586.3(2012):222-228.