Quantitative Proteomics Reveal up-regulated Protein Expression of the SET Complex Associated with Hepatocellular Carcinoma

	Quantitative Proteomics Reveal up-regulated Protein Expression of the SET Complex Associated with Hepatocellular Carcinoma
	Li, C; Ruan, HQ; Liu, YS; Xu, MJ; Dai, J; Sheng, QH; Tan, YX; Yao, ZZ; Wang, HY; Wu, JR
刊名	JOURNAL OF PROTEOME RESEARCH
	2012
卷号	11 期号:2 页码:871-885
关键词	hepatocellular carcinoma clinical tissues quantitative proteomics biomarkers the SET complex
通讯作者	Zeng, R (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn
英文摘要	We combined culture-derived isotope tags (CDITs) with two-dimensional liquid chromatography tandem mass spectrometry (2D-LC-MS/MS) to extensively survey abnormal protein expression associated with hepatocellular carcinoma (HCC) in clinical tissues. This approach yielded an in-depth quantitated proteome of 1360 proteins. Importantly, 267 proteins were significantly regulated with a fold-change of at least 1.5. The proteins up-regulated in HCC tissues are involved in regulatory processes, such as the granzyme A-mediated apoptosis pathway (The GzmA pathway). The SET complex, a central component in the GzmA pathway, was significantly up-regulated in HCC tissue. The elevated expressions of all of the SET complex components were validated by Western blotting. Among them, ANP32A and APEX1 were further investigated by immunohistochemistry staining using tissue microarrays (59 cases), confirming their overexpression in tumors. The up-regulation and nuclear accumulations of APEX1 was associated not only with HCC malignancy but also with HCC differentiation in 96 clinical HCC cases. Our work provided a systematic and quantitative analysis and demonstrated key changes in clinical HCC tissues. These proteomic signatures could help to unveil the underlying mechanisms of hepatocarcinogenesis and may be useful for the discovery of candidate biomarkers.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	HEPATITIS-B-VIRUS ; MASS-SPECTROMETRY IDENTIFICATION ; LASER CAPTURE MICRODISSECTION ; HUMAN PLASMA PROTEOME ; GRANZYME-A ; NUCLEAR PHOSPHOPROTEIN ; CELL-CULTURE ; AMINO-ACIDS ; CANCER ; SILAC
收录类别	SCI
语种	英语
WOS记录号	WOS:000300458300031
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/549]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, C,Ruan, HQ,Liu, YS,et al. Quantitative Proteomics Reveal up-regulated Protein Expression of the SET Complex Associated with Hepatocellular Carcinoma[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(2):871-885.
APA	Li, C.,Ruan, HQ.,Liu, YS.,Xu, MJ.,Dai, J.,...&Zeng, R.(2012).Quantitative Proteomics Reveal up-regulated Protein Expression of the SET Complex Associated with Hepatocellular Carcinoma.JOURNAL OF PROTEOME RESEARCH,11(2),871-885.
MLA	Li, C,et al."Quantitative Proteomics Reveal up-regulated Protein Expression of the SET Complex Associated with Hepatocellular Carcinoma".JOURNAL OF PROTEOME RESEARCH 11.2(2012):871-885.