In vivo site-specific integration of transgene in silkworm via PhiC31 integrase-mediated cassette exchange

	In vivo site-specific integration of transgene in silkworm via PhiC31 integrase-mediated cassette exchange
	Long, DP; Zhao, AC; Xu, LX; Lu, WJ; Guo, Q; Zhang, Y; Xiang, ZH
刊名	INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
	2013
卷号	43 期号:11 页码:997-1008
关键词	Site-specific integration phiC31/att system Bombyx mori piggyBac Transgenic silkworm
通讯作者	Zhao, AC (reprint author), Southwest Univ, State Key Lab Silkworm Genome Biol, Key Lab Sericulture Funct Genom & Biotechnol, Minist Agr, Chongqing 400716, Peoples R China.,zhaoaichun@hotmail.com
英文摘要	Current techniques for genetic engineering of the silkworm Bombyx mori genome utilize transposable elements, which result in positional effects and insertional mutagenesis through random insertion of exogenous DNA. New methods for introducing transgenes at specific positions are therefore needed to overcome the limitations of transposon-based strategies. Although site-specific recombination systems have proven powerful tools for genome manipulation in many organisms, their use has not yet been well established for the integration of transgenes in the silkworm. We describe a method for integrating target genes at pre-defined chromosomal sites in the silkworm via phiC31/att site-specific recombination system-mediated cassette exchange. Successful recombinase-mediated cassette exchange (RMCE) was observed in the two transgenic target strains with an estimated transformation efficiency of 3.84-7.01%. Our results suggest that RMCE events between chromosomal attP/attP target sites and incoming attB/attB sites were more frequent than those in the reciprocal direction. This is the first report of in vivo RMCE via phiC31 integrase in the silkworm, and thus represents a key step toward establishing genome manipulation technologies in silkworms and other lepidopteran species. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Entomology
类目[WOS]	Biochemistry & Molecular Biology ; Entomology
关键词[WOS]	PHI-C31 INTEGRASE ; BOMBYX-MORI ; HIGH-EFFICIENCY ; DROSOPHILA-MELANOGASTER ; GERMLINE TRANSFORMATION ; PHAGE PHI-C31 ; AEDES-AEGYPTI ; GENOME ; RECOMBINATION ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000326429600002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/445]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Long, DP,Zhao, AC,Xu, LX,et al. In vivo site-specific integration of transgene in silkworm via PhiC31 integrase-mediated cassette exchange[J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,2013,43(11):997-1008.
APA	Long, DP.,Zhao, AC.,Xu, LX.,Lu, WJ.,Guo, Q.,...&Xiang, ZH.(2013).In vivo site-specific integration of transgene in silkworm via PhiC31 integrase-mediated cassette exchange.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,43(11),997-1008.
MLA	Long, DP,et al."In vivo site-specific integration of transgene in silkworm via PhiC31 integrase-mediated cassette exchange".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 43.11(2013):997-1008.