MiR-9 reduces human acyl-coenzyme A:cholesterol acyltransferase-1 to decrease THP-1 macrophage-derived foam cell formation

	MiR-9 reduces human acyl-coenzyme A:cholesterol acyltransferase-1 to decrease THP-1 macrophage-derived foam cell formation
	Xu, JJ; Hu, GJ; Lu, M; Xiong, Y; Li, Q; Chang, CCY; Song, BL; Chang, TY; Li, BL
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2013
卷号	45 期号:11 页码:953-962
关键词	microRNA ACAT1 cholesteryl ester foam cell atherosclerosis
通讯作者	Li, BL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,blli@sibcb.ac.cn
英文摘要	MicroRNAs (miRNAs) post-transcriptionally regulate gene expression by targeting mRNAs and control a wide range of biological functions. Recent studies have indicated that miRNAs can regulate lipid and cholesterol metabolism in mammals. Acyl-coenzyme A:cholesterol acyltransferase (ACAT) is a key enzyme in cellular cholesterol metabolism. The accumulated cholesteryl esters are mainly synthesized by ACAT1 during the formation of foam cell, a hallmark of early atherosclerotic lesions. Here, we revealed that miR-9 could target the 3-untranslated region of human ACAT1 mRNA, specifically reduce human ACAT1 or reporter firefly luciferase (Fluc) proteins but not their mRNAs in different human cell lines, and functionally decrease the formation of foam cells from THP-1-derived macrophages. Our findings suggest that miR-9 might be an important regulator in cellular cholesterol homeostasis and decrease the formation of foam cells in vivo by reducing ACAT1 proteins.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	2 DIFFERENT CHROMOSOMES ; MESSENGER-RNA DECAY ; CHOLESTEROL ACYLTRANSFERASE ; GENE-EXPRESSION ; IN-VIVO ; MICRORNAS ; ATHEROSCLEROSIS ; PROTEIN ; INFLAMMATION ; HOMEOSTASIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000326288800008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/444]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, JJ,Hu, GJ,Lu, M,et al. MiR-9 reduces human acyl-coenzyme A:cholesterol acyltransferase-1 to decrease THP-1 macrophage-derived foam cell formation[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2013,45(11):953-962.
APA	Xu, JJ.,Hu, GJ.,Lu, M.,Xiong, Y.,Li, Q.,...&Li, BL.(2013).MiR-9 reduces human acyl-coenzyme A:cholesterol acyltransferase-1 to decrease THP-1 macrophage-derived foam cell formation.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,45(11),953-962.
MLA	Xu, JJ,et al."MiR-9 reduces human acyl-coenzyme A:cholesterol acyltransferase-1 to decrease THP-1 macrophage-derived foam cell formation".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 45.11(2013):953-962.