Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development

	Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development
	Chen, PF; Zhang, J; Zhan, Y; Su, JJ; Du, YR; Xu, GL; Shi, YF; Siebenlist, U; Zhang, XR
刊名	PLOS ONE
	2013
卷号	8 期号:9 页码:42006
通讯作者	Zhang, XR (reprint author), Chinese Acad Sci, Key Lab Stem Cell Biol, Inst Hlth Sci, Shanghai, Peoples R China.,xrzhang@sibs.ac.cn
英文摘要	Common thymic epithelial progenitor/stem cells (TEPCs) differentiate into cortical and medullary thymic epithelial cells (TECs), which are required for the development and selection of thymocytes. Mature TEC lines have been widely established. However, the establishment of TEPC lines is rarely reported. Here we describe the establishment of thymic epithelial stomal cell lines, named TSCs, from fetal thymus. TSCs express some of the markers present on tissue progenitor/stem cells such as Sca-1. Gene expression profiling verifies the thymic identity of TSCs. RANK stimulation of these cells induces expression of autoimmune regulator (Aire) and Aire-dependent tissue-restricted antigens (TRAs) in TSCs in vitro. TSCs could be differentiated into medullary thymic epithelial cell-like cells with exogenously expressed NF-kappa B subunits ReIB and p52. Importantly, upon transplantation under the kidney capsules of nude mice, TSCs are able to differentiate into mature TEC-like cells that can support some limited development of T cells in vivo. These findings suggest that the TSC lines we established bear some characteristics of TEPC cells and are able to differentiate into functional TEC-like cells in vitro and in vivo. The cloned TEPC-like cell lines may provide useful tools to study the differentiation of mature TEC cells from precursors.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	EMBRYONIC STEM-CELLS ; AUTOIMMUNE REGULATOR ; NEGATIVE SELECTION ; SELF-TOLERANCE ; AIRE ; MICROENVIRONMENT ; THYMOCYTES ; IDENTIFICATION ; PROLIFERATION ; MAINTENANCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000326520200090
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/424]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, PF,Zhang, J,Zhan, Y,et al. Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development[J]. PLOS ONE,2013,8(9):42006.
APA	Chen, PF.,Zhang, J.,Zhan, Y.,Su, JJ.,Du, YR.,...&Zhang, XR.(2013).Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development.PLOS ONE,8(9),42006.
MLA	Chen, PF,et al."Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development".PLOS ONE 8.9(2013):42006.