beta-Arrestin-1 directly interacts with Gas and regulates its function

	beta-Arrestin-1 directly interacts with Gas and regulates its function
	Li, B; Wang, CC; Zhou, ZC; Zhao, J; Pei, G
刊名	FEBS LETTERS
	2013
卷号	587 期号:5 页码:410-416
关键词	beta-Arrestin-1 G protein Interaction Biolayer interferometry analysis GTP gamma S binding and release
通讯作者	Zhao, J (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jzhao@sibs.ac.cn ; gpei@sibs.ac.cn
英文摘要	beta-Arrestins function to mediate G protein-coupled receptor (GPCR) desensitization and internalization and to initiate G protein independent signaling of GPCRs. Elucidating how beta-arrestin and G protein signal pathways coordinate with each other is important to fully understand GPCR signaling. Here we report that beta-arrestin-1 directly interacts with G alpha(s). Purified beta-arrestin-1 binds to G alpha(s) in a rapid association and dissociation manner. beta-Arrestin-1 promotes the binding and the release of GTP gamma S from G alpha(s) in vitro. beta-Arrestin-1 L33K mutant shows reduced interaction with G alpha(s) and has no detectable effects on G alpha(s) function. Our study thus reveals a direct crosstalk of beta-arrestin-1 with G alpha(s). Structured summary of protein interactions: G alpha q physically interacts with Beta-arrestin-1 by cross-linking study (View interaction). G alpha q physically interacts with Beta-arrestin-1 by anti tag coimmunoprecipitation (View interaction). G alpha s binds to Beta-arrestin-1 by biophysical (View Interaction: 1, 2). G alpha s physically interacts with Beta-arrestin-1 by anti tag coimmunoprecipitation (View interaction). Beta-arrestin-1 physically interacts with Gas by anti bait coimmunoprecipitation (View interaction). G alpha s physically interacts with Beta-arrestin-1 by cross-linking study (View interaction). (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	NUCLEOTIDE EXCHANGE FACTOR ; HETEROTRIMERIC G-PROTEINS ; BETA-ARRESTIN ; BETA(2)-ADRENERGIC RECEPTOR ; CRYSTAL-STRUCTURE ; GTPASE ACTIVITY ; ALPHA-SUBUNITS ; ACTIVATION ; BINDING ; MECHANISM
收录类别	SCI
语种	英语
WOS记录号	WOS:000315217900005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/392]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, B,Wang, CC,Zhou, ZC,et al. beta-Arrestin-1 directly interacts with Gas and regulates its function[J]. FEBS LETTERS,2013,587(5):410-416.
APA	Li, B,Wang, CC,Zhou, ZC,Zhao, J,&Pei, G.(2013).beta-Arrestin-1 directly interacts with Gas and regulates its function.FEBS LETTERS,587(5),410-416.
MLA	Li, B,et al."beta-Arrestin-1 directly interacts with Gas and regulates its function".FEBS LETTERS 587.5(2013):410-416.