Overexpression of hnRNPC2 induces multinucleation by repression of Aurora B in hepatocellular carcinoma cells

	Overexpression of hnRNPC2 induces multinucleation by repression of Aurora B in hepatocellular carcinoma cells
	Sun, DQ; Wang, Y; Liu, DG
刊名	ONCOLOGY LETTERS
	2013
卷号	5 期号:4 页码:1243-1249
关键词	heterogeneous ribonuclear protein C2 multinucleation hepatocellular carcinoma cell Aurora B eukaryotic translational initiation factor 4E
通讯作者	Liu, DG (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,dgliu@sibs.ac.cn
英文摘要	Heterogeneous ribonuclear protein C2 (hnRNPC2), an RNA binding protein, is a component of hnRNPC which is upregulated in many tumors. Multinucleation exists in many tumors and is positively correlated with tumor grade. To uncover the correlation between hnRNPC2 and multinucleation in hepatocellular carcinoma SMMC-7721 cells, we constructed a pEGFP-hnRNPC2 vector and transfected it into cancer cells. Our results revealed that overexpression of hnRNPC2 induced multinucleation in SMMC-7721 cells. Tracking tests indicated that the induced multinucleated cells were unable to recover to mononuclear cells and finally died as a result of defects in cell division. Furthermore, Aurora B, which was localized at the midbody and plays a role in cytokinesis, was repressed in hnRNPC2-overexpressing cells, whose knockdown by RNA interference also induced multinucleation in SMMC-7721 cells. Quantitative polymerase chain reaction (qPCR) and mRNA-protein co-immunoprecipitation results revealed that Aurora B mRNA did not decrease in hnRNPC2-overexpressing cells, instead it bound more hnRNPC2 and less eIF4E, an mRNA cap binding protein and translational initiation factor. Moreover, hnRNPC2 bound more eIF4E in hnRNPC2-overexpressing cells. These results indicate that hnRNPC2 repressed Aurora B binding with eIF4F, which must bind with Aurora B mRNA in order to initiate its translation. This induced multinucleation in hepatocellular carcinoma cells. In addition, hnRNPC2 accelerated hepatocellular carcinoma cell proliferation. Collectively, these data suggest that hnRNPC2 may be a potential target for hepatocellular carcinoma cell diagnosis and treatment.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	MESSENGER-RNA ; KINASE-ACTIVITY ; POOR-PROGNOSIS ; PROTEIN ; CYTOKINESIS ; CANCER ; EXPRESSION ; PROLIFERATION ; TRANSLATION ; PHOSPHORYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000321003100028
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/376]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Sun, DQ,Wang, Y,Liu, DG. Overexpression of hnRNPC2 induces multinucleation by repression of Aurora B in hepatocellular carcinoma cells[J]. ONCOLOGY LETTERS,2013,5(4):1243-1249.
APA	Sun, DQ,Wang, Y,&Liu, DG.(2013).Overexpression of hnRNPC2 induces multinucleation by repression of Aurora B in hepatocellular carcinoma cells.ONCOLOGY LETTERS,5(4),1243-1249.
MLA	Sun, DQ,et al."Overexpression of hnRNPC2 induces multinucleation by repression of Aurora B in hepatocellular carcinoma cells".ONCOLOGY LETTERS 5.4(2013):1243-1249.