MicroRNA-mediated repression of nonsense mRNAs

	MicroRNA-mediated repression of nonsense mRNAs
	Zhao, Y; Lin, JM; Xu, BY; Hu, SD; Zhang, X; Wu, LG
刊名	ELIFE
	2014
卷号	3 期号:1 页码:42006
通讯作者	Wu, LG (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,lgwu@sibcb.ac.cn
英文摘要	Numerous studies have established important roles for microRNAs (miRNAs) in regulating gene expression. Here, we report that miRNAs also serve as a surveillance system to repress the expression of nonsense mRNAs that may produce harmful truncated proteins. Upon recognition of the premature termination codon by the translating ribosome, the downstream portion of the coding region of an mRNA is redefined as part of the 3' untranslated region; as a result, the miRNA-responsive elements embedded in this region can be detected by miRNAs, triggering accelerated mRNA deadenylation and translational inhibition. We demonstrate that naturally occurring cancer-causing APC (adenomatous polyposis coli) nonsense mutants which escape nonsense-mediated mRNA decay (NMD) are repressed by miRNA-mediated surveillance. In addition, we show that miRNA-mediated surveillance and exon-exon junction complex-mediated NMD are not mutually exclusive and act additively to enhance the repressive activity. Therefore, we have uncovered a new role for miRNAs in repressing nonsense mutant mRNAs.
学科主题	Life Sciences & Biomedicine - Other Topics
类目[WOS]	Biology
关键词[WOS]	INTRON RETENTION ; CODING REGIONS ; OVARIAN-CANCER ; APC MUTATIONS ; COLORECTAL TUMORS ; BINDING-PROTEIN ; HUMAN-CELLS ; C-FOS ; DECAY ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000341250400002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/296]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, Y,Lin, JM,Xu, BY,et al. MicroRNA-mediated repression of nonsense mRNAs[J]. ELIFE,2014,3(1):42006.
APA	Zhao, Y,Lin, JM,Xu, BY,Hu, SD,Zhang, X,&Wu, LG.(2014).MicroRNA-mediated repression of nonsense mRNAs.ELIFE,3(1),42006.
MLA	Zhao, Y,et al."MicroRNA-mediated repression of nonsense mRNAs".ELIFE 3.1(2014):42006.