A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading

	A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading
	Zhou, XL; Ruan, ZR; Wang, M; Fang, ZP; Wang, Y; Chen, Y; Liu, RJ; Eriani, G; Wang, ED
刊名	NUCLEIC ACIDS RESEARCH
	2014
卷号	42 期号:22 页码:13873-13886
通讯作者	Wang, ED (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai, Peoples R China.,edwang@sibs.ac.cn
英文摘要	Yeast mitochondria contain a minimalist threonyl-tRNA synthetase (ThrRS) composed only of the catalytic core and tRNA binding domain but lacking the entire editing domain. Besides the usual tRNA(Thr)2, some budding yeasts, such as Saccharomyces cerevisiae, also contain a non-canonical tRNA(Thr)1 with an enlarged 8-nucleotide anticodon loop, reprograming the usual leucine CUN codons to threonine. This raises interesting questions about the aminoacylation fidelity of such ThrRSs and the possible contribution of the two tRNA(Thr)s during editing. Here, we found that, despite the absence of the editing domain, S. cerevisiae mitochondrial ThrRS (ScmtThrRS) harbors a tRNA-dependent pre-transfer editing activity. Remarkably, only the usual tRNA(Thr)2 stimulated pre-transfer editing, thus, establishing the first example of a synthetase exhibiting tRNA-isoacceptor specificity during pre-transfer editing. We also showed that the failure of tRNAThr1 to stimulate tRNA-dependent pre-transfer editing was due to the lack of an editing domain. Using assays of the complementation of a ScmtThrRS gene knockout strain, we showed that the catalytic core and tRNA binding domain of ScmtThrRS co-evolved to recognize the unusual tRNA(Thr)1. In combination, the results provide insights into the tRNA-dependent editing process and suggest that tRNA-dependent pretransfer editing takes place in the aminoacylation catalytic core.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	PROTEIN-SYNTHESIS ; QUALITY-CONTROL ; SACCHAROMYCES-CEREVISIAE ; TRANSLATIONAL FIDELITY ; YEAST MITOCHONDRIA ; DOMAIN ; MECHANISM ; SEQUENCE ; ARCHAEA ; GENOME
收录类别	SCI
语种	英语
WOS记录号	WOS:000347916900043
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/263]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, XL,Ruan, ZR,Wang, M,et al. A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading[J]. NUCLEIC ACIDS RESEARCH,2014,42(22):13873-13886.
APA	Zhou, XL.,Ruan, ZR.,Wang, M.,Fang, ZP.,Wang, Y.,...&Wang, ED.(2014).A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading.NUCLEIC ACIDS RESEARCH,42(22),13873-13886.
MLA	Zhou, XL,et al."A minimalist mitochondrial threonyl-tRNA synthetase exhibits tRNA-isoacceptor specificity during proofreading".NUCLEIC ACIDS RESEARCH 42.22(2014):13873-13886.