The Specification and Global Reprogramming of Histone Epigenetic Marks during Gamete Formation and Early Embryo Development in C. elegans

	The Specification and Global Reprogramming of Histone Epigenetic Marks during Gamete Formation and Early Embryo Development in C. elegans
	Samson, M; Jow, MM; Wong, CCL; Fitzpatrick, C; Aslanian, A; Saucedo, I; Estrada, R; Ito, T; Park, SKR; Yates, JR
刊名	PLOS GENETICS
	2014
卷号	10 期号:10 页码:e1004588-e1004588
通讯作者	Samson, M (reprint author), San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA.,chud@sfsu.edu
英文摘要	In addition to the DNA contributed by sperm and oocytes, embryos receive parent-specific epigenetic information that can include histone variants, histone post-translational modifications (PTMs), and DNA methylation. However, a global view of how such marks are erased or retained during gamete formation and reprogrammed after fertilization is lacking. To focus on features conveyed by histones, we conducted a large-scale proteomic identification of histone variants and PTMs in sperm and mixed-stage embryo chromatin from C. elegans, a species that lacks conserved DNA methylation pathways. The fate of these histone marks was then tracked using immunostaining. Proteomic analysis found that sperm harbor similar to 2.4 fold lower levels of histone PTMs than embryos and revealed differences in classes of PTMs between sperm and embryos. Sperm chromatin repackaging involves the incorporation of the sperm-specific histone H2A variant HTAS-1, a widespread erasure of histone acetylation, and the retention of histone methylation at sites that mark the transcriptional history of chromatin domains during spermatogenesis. After fertilization, we show HTAS-1 and 6 histone PTM marks distinguish sperm and oocyte chromatin in the new embryo and characterize distinct paternal and maternal histone remodeling events during the oocyte-to-embryo transition. These include the exchange of histone H2A that is marked by ubiquitination, retention of HTAS-1, removal of the H2A variant HTZ-1, and differential reprogramming of histone PTMs. This work identifies novel and conserved features of paternal chromatin that are specified during spermatogenesis and processed in the embryo. Furthermore, our results show that different species, even those with diverged DNA packaging and imprinting strategies, use conserved histone modification and removal mechanisms to reprogram epigenetic information.
学科主题	Genetics & Heredity
类目[WOS]	Genetics & Heredity
关键词[WOS]	CAENORHABDITIS-ELEGANS ; SHOTGUN PROTEOMICS ; MASS-SPECTROMETRY ; GERM-LINE ; MOUSE SPERMATOGENESIS ; CORE HISTONES ; POSTTRANSLATIONAL MODIFICATIONS ; MAMMALIAN SPERMIOGENESIS ; DROSOPHILA-MELANOGASTER ; MEIOTIC MATURATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000344650700005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/229]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Samson, M,Jow, MM,Wong, CCL,et al. The Specification and Global Reprogramming of Histone Epigenetic Marks during Gamete Formation and Early Embryo Development in C. elegans[J]. PLOS GENETICS,2014,10(10):e1004588-e1004588.
APA	Samson, M.,Jow, MM.,Wong, CCL.,Fitzpatrick, C.,Aslanian, A.,...&Chu, DS.(2014).The Specification and Global Reprogramming of Histone Epigenetic Marks during Gamete Formation and Early Embryo Development in C. elegans.PLOS GENETICS,10(10),e1004588-e1004588.
MLA	Samson, M,et al."The Specification and Global Reprogramming of Histone Epigenetic Marks during Gamete Formation and Early Embryo Development in C. elegans".PLOS GENETICS 10.10(2014):e1004588-e1004588.