Expression profiles of histone lysine demethylases during cardiomyocyte differentiation of mouse embryonic stem cells

	Expression profiles of histone lysine demethylases during cardiomyocyte differentiation of mouse embryonic stem cells
	Tang, Y; Chen, ZY; Hong, YZ; Wu, Q; Lin, HQ; Chen, CD; Yang, HT
刊名	ACTA PHARMACOLOGICA SINICA
	2014
卷号	35 期号:7 页码:899-906
关键词	embryonic stem cell cardiac differentiation cardiomyocyte histone lysine demethylase expression profile Brachyury Flk-1(+)/Cxcr4(+)
通讯作者	Yang, HT (reprint author), Shanghai Jiao Tong Univ, Inst Hlth Sci, Key Lab Stem Cell Biol, Sch Med,SIBS,CAS, Shanghai 200031, Peoples R China.,htyang@sibs.ac.cn
英文摘要	Aim: Histone lysine demethylases (KDMs) control the lineage commitments of stem cells. However, the KDMs involved in the determination of the cardiomyogenic lineage are not fully defined. The aim of this study was to investigate the expression profiles of KDMs during the cardiac differentiation of mouse embryonic stem cells (mESCs). Methods: An in vitro cardiac differentiation system of mESCs with Brachyury (a mesodermal specific marker) and Flk-1(+)/Cxcr4(+) (dual cell surface biomarkers) selection was used. The expression profiles of KDMs during differentiation were analyzed using Q-PCR. To understand the contributions of KDMs to cardiomyogenesis, the mESCs on differentiation d 3.5 were sorted by FACS into Brachyury(+) cells and Brachyury(-) cells, and mESCs on d 5.5 were sorted into Flk-1(+)/Cxcr4(+) and Flk-1(-)/Cxcr4(-) cells. Results: mESCs were differentiated into spontaneously beating cardiomyocytes that were visible in embryoid bodies (EBs) on d 7. On d 12-14, all EBs developed spontaneously beating cardiomyocytes. Among the 16 KDMs examined, the expression levels of Phf8, Jarid1a, Jhdm1d, Utx, and Jmjd3 were increased by nearly 2-6-fold on d 14 compared with those on d 0. Brachyury(+) cells showed higher expression levels of Jmjd3, Jmjd2a and Jhdm1d than Brachyury- cells. A higher level of Jmjd3 was detected in Flk-1(+)/Cxcr4(+) cells, whereas the level of Jmjd2c was lower in both Brachyury(+) cells and Flk-1(+)/Cxcr4(+) cells. Conclusion: KDMs may play important roles during cardiomyogenesis of mESCs. Our results provide a clue for further exploring the roles of KDMs in the cardiac lineage commitment of mESCs and the potential interference of cardiommenesis.
学科主题	Chemistry; Pharmacology & Pharmacy
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
关键词[WOS]	CARDIAC PROGENITOR CELLS ; SELF-RENEWAL ; DEVELOPMENTAL GENES ; IN-VITRO ; PLURIPOTENT ; TRANSCRIPTION ; LINEAGE ; UTX ; INDUCTION ; PROSPECTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000338910500006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/211]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Tang, Y,Chen, ZY,Hong, YZ,et al. Expression profiles of histone lysine demethylases during cardiomyocyte differentiation of mouse embryonic stem cells[J]. ACTA PHARMACOLOGICA SINICA,2014,35(7):899-906.
APA	Tang, Y.,Chen, ZY.,Hong, YZ.,Wu, Q.,Lin, HQ.,...&Yang, HT.(2014).Expression profiles of histone lysine demethylases during cardiomyocyte differentiation of mouse embryonic stem cells.ACTA PHARMACOLOGICA SINICA,35(7),899-906.
MLA	Tang, Y,et al."Expression profiles of histone lysine demethylases during cardiomyocyte differentiation of mouse embryonic stem cells".ACTA PHARMACOLOGICA SINICA 35.7(2014):899-906.