Structure of MST2 SARAH domain provides insights into its interaction with RAPL

	Structure of MST2 SARAH domain provides insights into its interaction with RAPL
	Liu, GG; Shi, ZB; Jiao, S; Zhang, ZZ; Wang, WJ; Chen, CC; Hao, Q; Zhang, M; Feng, M; Xu, L
刊名	JOURNAL OF STRUCTURAL BIOLOGY
	2014
卷号	185 期号:3 页码:366-374
关键词	MST2 RAPL/RASSF5/NORE1 SARAH domain Coiled coil Apoptosis
通讯作者	Zhou, ZC (reprint author), Inst Biochem & Cell Biol, State Key Lab Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,zczhou@sibcb.ac.cn ; zhmin07@ustc.edu
英文摘要	The STE20 kinases MST1 and MST2 are key players in mammalian Hippo pathway. The SARAH domains of MST1/2 act as a platform to mediate homodimerization and hetero-interaction with a range of adaptors including RASSFs and Salvador, which also possess SARAH domains. Here, we determined the crystal structure of human MST2 SARAH domain, which forms an antiparallel homodimeric coiled coil. Structural comparison indicates that SARAH domains of different proteins may utilize a shared dimerization module to form homodimer or heterodimer. Structure-guided mutational study identified specific interface residues critical for MST2 homodimerization. MST2 mutations disrupting its homodimerization also impaired its hetero-interaction with RAPL (also named RASSF5 and NORE1), which is mediated by their SARAH domains. Further biochemical and cellular assays indicated that SARAH domain-mediated homodimerization and hetero-interaction with RAPL are required for full activation of MST2 and therefore apoptotic functions in T cells. (C) 2014 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	PROMOTES APOPTOSIS ; CELL-PROLIFERATION ; PROTEIN-KINASE ; HIPPO ; PATHWAY ; RAF-1 ; SUPPRESSION ; ACTIVATION ; EGRESS ; ROLES
收录类别	SCI
语种	英语
WOS记录号	WOS:000332815400015
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/163]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, GG,Shi, ZB,Jiao, S,et al. Structure of MST2 SARAH domain provides insights into its interaction with RAPL[J]. JOURNAL OF STRUCTURAL BIOLOGY,2014,185(3):366-374.
APA	Liu, GG.,Shi, ZB.,Jiao, S.,Zhang, ZZ.,Wang, WJ.,...&Zhang, M.(2014).Structure of MST2 SARAH domain provides insights into its interaction with RAPL.JOURNAL OF STRUCTURAL BIOLOGY,185(3),366-374.
MLA	Liu, GG,et al."Structure of MST2 SARAH domain provides insights into its interaction with RAPL".JOURNAL OF STRUCTURAL BIOLOGY 185.3(2014):366-374.