A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse

	A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse
	Liu, C; Zhao, XW; Xu, LL; Yi, JY; Shaheen, S; Han, WD; Wang, F; Zheng, WJ; Xu, CQ; Liu, WL
刊名	JOURNAL OF LEUKOCYTE BIOLOGY
	2015
卷号	97 期号:5 页码:887-900
关键词	activation Btk
通讯作者	Liu, WL (reprint author), Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China.,liuwanli@biomed.tsinghua.edu.cn
英文摘要	Advanced live cell imaging studies suggested that B cell activation is initiated by the formation of BCR microclusters and subsequent B cell IS upon BCR and antigen recognition. PKC family member PKC beta is highly expressed in B cells and plays an important role in the initiation of B cell activation. Here, we reported an inhibitory function of PKC beta through a negative-feedback manner in B cell activation. Compared with WT (PKC beta-WT) or the constitutively active (PKC beta-DNPS) form of PKC beta, DN PKC beta (PKC beta-DN) unexpectedly enhanced the accumulation of BCR microclusters into the B cell IS, leading to the recruitment of an excessive amount of pSyk, pPLC-gamma 2, and pBLNK signaling molecules into the membrane-proximal BCR signalosome. Enhanced calcium mobilization responses in the decay phase were also observed in B cells expressing PKC beta-DN. Mechanistic studies showed that this negative-feedback function of PKC beta works through the induction of an inhibitory form of pBtk at S180 (pBtk-S180). Indeed, the capability of inducing the formation of an inhibitory pBtk-S180 is in the order of PKC beta-DNPS. PKC beta-WT. PKC beta-DN. Thus, these results improve our comprehensive understanding on the positive and negative function of PKC beta in the fine tune of B cell activation.
学科主题	Cell Biology; Hematology; Immunology
类目[WOS]	Cell Biology ; Hematology ; Immunology
关键词[WOS]	PROTEIN-KINASE-C ; BRUTONS TYROSINE KINASE ; ANTIGEN RECEPTOR ; T-CELLS ; ACTIVATION ; EXPRESSION ; BTK ; AGAMMAGLOBULINEMIA ; POLARIZATION ; INITIATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000353900500007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/66]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, C,Zhao, XW,Xu, LL,et al. A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse[J]. JOURNAL OF LEUKOCYTE BIOLOGY,2015,97(5):887-900.
APA	Liu, C.,Zhao, XW.,Xu, LL.,Yi, JY.,Shaheen, S.,...&Liu, WL.(2015).A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse.JOURNAL OF LEUKOCYTE BIOLOGY,97(5),887-900.
MLA	Liu, C,et al."A negative-feedback function of PKC beta in the formation and accumulation of signaling-active B cell receptor microclusters within B cell immunological synapse".JOURNAL OF LEUKOCYTE BIOLOGY 97.5(2015):887-900.