Epididymal Region-Specific miRNA Expression and DNA Methylation and Their Roles in Controlling Gene Expression in Rats

	Epididymal Region-Specific miRNA Expression and DNA Methylation and Their Roles in Controlling Gene Expression in Rats
	Chu, C; Zheng, GY; Hu, SG; Zhang, JS; Xie, SS; Ma, WB; Ni, MJ; Tang, CH; Zhou, L; Zhou, YC
刊名	PLOS ONE
	2015
卷号	10 期号:4 页码:e0124450-e0124450
通讯作者	Li, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Syst Biol, Shanghai 200031, Peoples R China.,yxli@sibs.ac.cn ; ylzhang@sibcb.ac.cn
英文摘要	Region-specific gene expression is an intriguing feature of the mammalian epididymis. This unique property is essential for sperm maturation and storage, and it also implicates stringent and multi-level regulations of gene expression. Over the past decade, the androgen-driven activation of epididymal gene transcription has been extensively studied. However, it still remains largely unexplored whether and how other regulatory mechanisms, such as miRNAs and DNA methylation, are involved in controlling regional gene expression in the epididymis. Using microarray-based approaches, we studied the regional miRNA expression and DNA methylation profiles in 4 distinct epididymal regions (initial segment, caput, corpus and cauda) of rats. We found that the miR-200 family members were more expressed in caput, compared with cauda. By GSEA analysis, the differential expression of miR-200 family between caput and cauda was shown to be negatively correlated with their predicted target genes, among which 4 bona fide targets were verified by luciferase reporter assay. Predicted target genes of miR-200 family have enriched functions in anti-apoptosis, cell transportation and development, implying the regional diversity in epididymal functions. On the other hand, we revealed epididymal DNA methylation of 2002 CpG islands and 2771 gene promoters (-3.88-0.97kb), among which 1350 (67.43%) CpG islands and 2095 (75.60%) promoters contained region-specific DNA methylation. We observed significant and distinct functional enrichment in genes with specifically methylated promoters in each epididymal regions, but these DNA methylations did not show significant correlation with repressed gene transcription in the mature epididymis. Conclusively, we investigated the regional miRNA expression and DNA methylation in the rat epididymis and revealed a potential role of miR-200 family in gene expression regulation between caput and cauda. This may contribute to the distinct physiological function in sperm maturation / storage of caput / cauda epididymis.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	MIR-200 FAMILY ; MESSENGER-RNA ; E-CADHERIN ; MESENCHYMAL TRANSITION ; MOUSE EPIDIDYMIS ; REPRESSORS ZEB1 ; CANCER-CELLS ; STEM-CELLS ; MICRORNAS ; DIFFERENTIATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000353331500087
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/45]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chu, C,Zheng, GY,Hu, SG,et al. Epididymal Region-Specific miRNA Expression and DNA Methylation and Their Roles in Controlling Gene Expression in Rats[J]. PLOS ONE,2015,10(4):e0124450-e0124450.
APA	Chu, C.,Zheng, GY.,Hu, SG.,Zhang, JS.,Xie, SS.,...&Zhang, YL.(2015).Epididymal Region-Specific miRNA Expression and DNA Methylation and Their Roles in Controlling Gene Expression in Rats.PLOS ONE,10(4),e0124450-e0124450.
MLA	Chu, C,et al."Epididymal Region-Specific miRNA Expression and DNA Methylation and Their Roles in Controlling Gene Expression in Rats".PLOS ONE 10.4(2015):e0124450-e0124450.