Inhibition of Telomere Recombination by Inactivation of KEOPS Subunit Cgi121 Promotes Cell Longevity

	Inhibition of Telomere Recombination by Inactivation of KEOPS Subunit Cgi121 Promotes Cell Longevity
	Peng, J; He, MH; Duan, YM; Liu, YT; Zhou, JQ
刊名	PLOS GENETICS
	2015
卷号	11 期号:3 页码:e1005071-e1005071
通讯作者	Peng, J (reprint author), Univ Chinese Acad Sci, State Key Lab Mol Biol, Shanghai Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,Chinese Acad Sci, Shanghai, Peoples R China.,jqzhou@sibcb.ac.cn
英文摘要	DNA double strand break (DSB) is one of the major damages that cause genome instability and cellular aging. The homologous recombination (HR)-mediated repair of DSBs plays an essential role in assurance of genome stability and cell longevity. Telomeres resemble DSBs and are competent for HR. Here we show that in budding yeast Saccharomyces cerevisiae telomere recombination elicits genome instability and accelerates cellular aging. Inactivation of KEOPS subunit Cgi121 specifically inhibits telomere recombination, and significantly extends cell longevity in both telomerase-positive and pre-senescing telomerase-negative cells. Deletion of CGI121 in the short-lived yku80(tel) mutant restores lifespan to cgi121 Delta level, supporting the function of Cgi121 in telomeric single-stranded DNA generation and thus in promotion of telomere recombination. Strikingly, inhibition of telomere recombination is able to further slow down the aging process in long-lived fob1 Delta cells, in which rDNA recombination is restrained. Our study indicates that HR activity at telomeres interferes with telomerase to pose a negative impact on cellular longevity.
学科主题	Genetics & Heredity
类目[WOS]	Genetics & Heredity
关键词[WOS]	DOUBLE-STRAND BREAK ; REPLICATION FORK BLOCKING ; SACCHAROMYCES-CEREVISIAE ; LIFE-SPAN ; RIBOSOMAL DNA ; HOMOLOGOUS RECOMBINATION ; YEAST TELOMERASE ; KU HETERODIMER ; FOB1 PROTEIN ; EST GENES
收录类别	SCI
语种	英语
WOS记录号	WOS:000352197100050
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/29]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Peng, J,He, MH,Duan, YM,et al. Inhibition of Telomere Recombination by Inactivation of KEOPS Subunit Cgi121 Promotes Cell Longevity[J]. PLOS GENETICS,2015,11(3):e1005071-e1005071.
APA	Peng, J,He, MH,Duan, YM,Liu, YT,&Zhou, JQ.(2015).Inhibition of Telomere Recombination by Inactivation of KEOPS Subunit Cgi121 Promotes Cell Longevity.PLOS GENETICS,11(3),e1005071-e1005071.
MLA	Peng, J,et al."Inhibition of Telomere Recombination by Inactivation of KEOPS Subunit Cgi121 Promotes Cell Longevity".PLOS GENETICS 11.3(2015):e1005071-e1005071.