Exogenous CaCl2 promoted the indole alkaloid accumulation in seedlings of Catharanthus roseus under NaCl stress

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Exogenous CaCl2 promoted the indole alkaloid accumulation in seedlings of Catharanthus roseus under NaCl stress
	Liu, L.1; Wang, J. Y.1; Chen, F.1; Liu, C.2; Long, X. H.1; Liu, Z. P.1; Shao, H. B.1,3,4
刊名	PLANT BIOSYSTEMS
	2014-01-02
卷号	148 期号:1 页码:127-132
关键词	Catharanthus roseus alkaloids calcium chloride enzymes growth NaCl stress
ISSN号	1126-3504
通讯作者	Long, XH (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China. longxiaohua@njau.edu.cn ; shaohongbochu@126.com
产权排序	[Liu, L.; Wang, J. Y.; Chen, F.; Long, X. H.; Liu, Z. P.; Shao, H. B.] Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China; [Liu, C.] Inst Agr Sci Jiangsu Coastal Dist, Nanjing, Jiangsu, Peoples R China; [Shao, H. B.] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Beijing, Peoples R China; [Shao, H. B.] Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao, Shandong, Peoples R China
中文摘要	A sandy culture experiment was conducted to investigate the effects of exogenous CaCl2 on the indole alkaloid accumulation in Catharanthus roseus under salt stress. One-month seedlings of C. roseus were treated with the different concentrations of NaCl (0, 50, and 100mmoll(-1)) and 7.5mmoll(-1) CaCl2. The plant samples were analyzed after 7 days of the treatments. The NaCl-stressed plants showed decrease of fresh and dry weight and increase of malondialdehyde (MDA) content compared to control. Tryptophan decarboxylase (TDC) activity increased significantly under 50mmoll(-1) NaCl without CaCl2 addition, 50mmoll(-1) NaCl with 7.5mmoll(-1) CaCl2, and CaCl2 treatment without NaCl addition. There was a significant increase in peroxidase activity under NaCl stress compared to control. The vindoline, catharanthine, vincristine, and vinblastine contents increased under salt stress (especially with 50mmoll(-1) NaCl treatment with or without CaCl2). Addition of CaCl2 to NaCl-stressed plants increased biomass, TDC activity, vindoline, and catharanthine contents and lowered MDA and vincirstine contents compared to the plants without CaCl2. The plants treated with CaCl2 alone showed higher TDC activity, vindoline, catharanthine, and vinblastine content when compared to control. The results showed that exogenous CaCl2 could promote the indole alkaloid metabolism under salt stress.
英文摘要	A sandy culture experiment was conducted to investigate the effects of exogenous CaCl2 on the indole alkaloid accumulation in Catharanthus roseus under salt stress. One-month seedlings of C. roseus were treated with the different concentrations of NaCl (0, 50, and 100mmoll(-1)) and 7.5mmoll(-1) CaCl2. The plant samples were analyzed after 7 days of the treatments. The NaCl-stressed plants showed decrease of fresh and dry weight and increase of malondialdehyde (MDA) content compared to control. Tryptophan decarboxylase (TDC) activity increased significantly under 50mmoll(-1) NaCl without CaCl2 addition, 50mmoll(-1) NaCl with 7.5mmoll(-1) CaCl2, and CaCl2 treatment without NaCl addition. There was a significant increase in peroxidase activity under NaCl stress compared to control. The vindoline, catharanthine, vincristine, and vinblastine contents increased under salt stress (especially with 50mmoll(-1) NaCl treatment with or without CaCl2). Addition of CaCl2 to NaCl-stressed plants increased biomass, TDC activity, vindoline, and catharanthine contents and lowered MDA and vincirstine contents compared to the plants without CaCl2. The plants treated with CaCl2 alone showed higher TDC activity, vindoline, catharanthine, and vinblastine content when compared to control. The results showed that exogenous CaCl2 could promote the indole alkaloid metabolism under salt stress.
学科主题	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	WATER-DEFICIT STRESS ; SALT STRESS ; SALINITY STRESS ; AJMALICINE PRODUCTION ; ANTIOXIDANT ENZYMES ; PROLINE METABOLISM ; LIPID-PEROXIDATION ; OXIDATIVE STRESS ; DROUGHT STRESS ; GROWTH
收录类别	SCI
语种	英语
WOS记录号	WOS:000333880000018
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8563]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China 2.Inst Agr Sci Jiangsu Coastal Dist, Nanjing, Jiangsu, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Beijing, Peoples R China 4.Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, L.,Wang, J. Y.,Chen, F.,et al. Exogenous CaCl2 promoted the indole alkaloid accumulation in seedlings of Catharanthus roseus under NaCl stress[J]. PLANT BIOSYSTEMS,2014,148(1):127-132.
APA	Liu, L..,Wang, J. Y..,Chen, F..,Liu, C..,Long, X. H..,...&Shao, H. B..(2014).Exogenous CaCl2 promoted the indole alkaloid accumulation in seedlings of Catharanthus roseus under NaCl stress.PLANT BIOSYSTEMS,148(1),127-132.
MLA	Liu, L.,et al."Exogenous CaCl2 promoted the indole alkaloid accumulation in seedlings of Catharanthus roseus under NaCl stress".PLANT BIOSYSTEMS 148.1(2014):127-132.