A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity

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	A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity
	Wang, Leilei 1,2; Zhang, Ying 1,2; Wang, Lingling 1; Yang, Jialong 1,2; Zhou, Zhi 1,2; Gai, Yunchao 1,2; Qiu, Limei 1; Song, Linsheng 1
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY
	2011-12-05
卷号	10 期号:77 页码:17678-17689
关键词	Eriocheir sinensis anti-lipopolysaccharide factor Listonella anguillarum Bacillus subtilis antimicrobial activity
英文摘要	The cDNA of a new Eriocheir sinensis ALF (designated as EsALF-3) was obtained based on EST analysis. The full-length cDNA was of 956 bp, consisting of an open reading frame (ORF) of 369 bp encoding a polypeptide of 123 amino acids. In the deduced amino acid sequence of EsALF-3, there were two highly conserved cysteine residues to define the LPS binding site, and eight positively charged residues existed between the two cysteine residues. The phylogenetic analysis revealed that EsALF-3 was clustered with the ALFs from some crustaceans, forming a separate sister branch to the group of EsALF-1 and EsALF-2 from E. sinensis. The mRNA transcript of EsALF-3 was detected in all the examined tissues of crabs, including haemocytes, hepatopancreas, gill, muscle, heart and gonad. The recombinant protein of EsALF-3 represented antimicrobial activity against Listonella anguillarum, Escherichia coli and Bacillus subtilis with minimal inhibitory concentration (MIC) values of 33.75 mu g mL(-1), 270 mu g mL-1 and 135 mu g mL(-1), respectively. These results together indicated that there were multiplicate ALF isoforms coexist in E. sinensis, and the diversity of the immune effector molecules in innate immune system, such as ALF, helps Chinese mitten crab to deal with various pathogens in the aquatic environment.
收录类别	SCI
语种	英语
WOS记录号	WOS:000298556200004
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/32942]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Leilei,Zhang, Ying,Wang, Lingling,et al. A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(77):17678-17689.
APA	Wang, Leilei.,Zhang, Ying.,Wang, Lingling.,Yang, Jialong.,Zhou, Zhi.,...&Song, Linsheng.(2011).A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(77),17678-17689.
MLA	Wang, Leilei,et al."A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.77(2011):17678-17689.